Summary
The derivatization reaction of human plasma was performed with 2,4-dinitrofluorobenzen at 40 °C during 3 hours. Separation occurred on reverse-phase C-18 columns, 50 min were necessary to achieve complete elution.
Exactitude, precision, sensitivity of the method were better than the conventional ion-exchange separation and ninhydrin assay procedure.
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Bachmann, E.W., Frei, J. & Muehlemann, M. Determination of amino acids in human plasma by high-pressure liquid chromatography. Chromatographia 12, 345–348 (1979). https://doi.org/10.1007/BF02259151
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DOI: https://doi.org/10.1007/BF02259151