Summary
A number of coumarins, furocoumarins and pyranocoumarins were investigated using HPTLC and HPLC systems consisting of silica gel and binary and ternary solvents containing a polar modifier (acetonitrile, dioxane, diisopropyl ether, methyl ethyl ketone, ethyl acetate, 2-propanol or tetrahydrofuran) and a non-polar or weakly polar diluent (n-heptane or dichloromethane). The experimental results obtained on thin layers, under isocratic conditions, showed a linear relationship between the RM values and the log of the concentration of the polar modifier.
The influence of the modifier and the individual substituents in the solute molecule on retention is presented as chromatographic “spectra” by plotting the RM and logk′ values against the mobile phase used.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
O. Schimmer, Planta Med.47, 79 (1983).
E. Van Scott, J. Am. Med. Assoc.235, 197 (1976).
O. Thastrup, B. Fjalland, J. Lemmich, Acta Pharmacol. Toxicol.52, 246 (1983).
J. Karlsen, L. E. J. Boomsma, A. B. Svendsen, J. Chromatogr.42, 550 (1969).
T. Beyrich, J. Chromatogr.20, 173 (1965).
K. Wierzchowska-Renke, Acta Polon. Pharm.31, 225 (1974).
B. Kamiński, K. Glowniak, A. Majewska, J. Petkowicz, D. Szaniawska-Dekundy. Farm. Pol.34, 25 (1978).
C. F. Van Sumere, G. Wolf, H. Teuchy, J. Kint, J. Chromatogr.20, 48 (1965).
H. J. Thompson, S. A. Brown, J. Chromatogr.314, 323 (1984).
K. Vande Casteele, H. Geiger, C. F. Van Sumere, J. Chromatogr.258, 111 (1983).
F. D. Stermitz, R. D. Thomsa, J. Chromatogr.77, 431 (1973).
R. C. Beier, J. Liq. Chromatogr.8 (10), 1923 (1985).
R. G. Enriquez, M. L. Romero, L. I. Escobar, P. Joseph-Nathan, W. F. Reynolds, J. Chromatogr.287, 209 (1984).
C. A. J. Erdeimeier, B. Meier, O. Sticher, J. Chromatogr.346, 456 (1984).
K. Glowniak, M. Bieganowska, J. Liq. Chromatogr.8, (16), 2927 (1985).
D. G. Walter, B. G. Lake, R. C. Cottrell, J. Chromatogr.196, 501 (1980).
M. Kowalska, J. Skrzypczakowa, Dissert. Pharm. Cracow26, 225 (1964).
W. G. van der Sluis, J. van Arkel, F. C. Fischer, J. R. P. Labadie, J. Chromatogr.214, 349 (1981).
J. Mendez, J. Rubido, Planta Med.36, 219 (1979).
E. H. Oertly, R. C. Beier, C. W. Ivie, L. D. Rowe, Photochem.23, 439 (1984).
K. Glowniak, M. L. Bieganowska, J. Chromatogr.370, 281 (1986).
K. Glowniak, G. Matysik, M. L. Bieganowska, E. Soczewiński, Chromatographia22, 7 (1986).
T. M. Swager, J. H. Cardellina, Phytochem.24, 805 (1985).
K. Glowniak, T. Wawrzynowicz, E. Soczewiński, in Chromatography '85 (H. Kalasz andL. S. Ettre, Eds.), Akademiai Kiado, Budapest 1986, p. 251.
N. E. Nordly, S. Nagy, J. Chromatogr.207, 21 (1981).
R. V. Tamma, C. C. Miller, R. Everett, J. Chromatogr.322, 236 (1985).
E. Soczewiński, Anal. Chem.41, 179 (1969).
E. Soczewiński, W. Golkiewicz, Chromatographie4, 501 (1971).
L. R. Snyder, Principles of Adsorption Chromatography, Marcel Dekker, New York, 1968.
E. Soczewiński, Chromatographia11, 534 (1978).
E. Soczewiński, J. Chromatogr.130, 23 (1977).
P. Jandera, M. Janderova, J. J. Churaček, J. Chromatogr.148, 79 (1978).
E. Soczewiński, in Planar Chromatography, Vol. 1 (R. E. Kaiser, Ed.) Huthig Verlag, Heidelberg, 1986; p. 79.
E. Soczewiński, J. Kuczmierczyk, J. Chromatogr.150, 53 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bieganowska, M.L., Glowniak, K. Retention behaviour of some coumarins in normal-phase high-performance thin-layer and column chromatography. Chromatographia 25, 111–116 (1988). https://doi.org/10.1007/BF02259026
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02259026