Skip to main content
SpringerLink
Log in

Usefulness of Thin-Layer Chromatography for the Prediction of High-Performance Liquid Chromatographic Retention Behavior of New 1,2,4-Triazole and Thiosemicarbazide Derivatives

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Summary

The purpose of this paper was to assess the suitability of thin-layer chromatography (TLC) as a pilot method for high-performance liquid chromatography (HPLC). The new 1,2,4-triazole and thiosemicarbazide derivatives were analyzed by use of reversed-phase (RP)-TLC and RP-HPLC techniques, applying octadecyl silica adsorbents and aqueous—organic mobile phases. Organic modifiers: methanol and acetonitrile were used at appropriate concentrations providing optimal retention factors. For both techniques, retention coefficient k in column chromatography and the retardation factor RF expressing retention in TLC were determined. The experimentally measured parameters were presented in a logarithmic scale as log k and RM, respectively. The constructed relationships between log k and RM allowed determining the possibility of TLC for anticipating HPLC retention behavior.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. Dobosz, M. Pitucha, M. Wujec, Acta Pol. Pharm. Drug Res. 53 (1996) 31–38.

    CAS  Google Scholar 

  2. M. Dobosz, M. Struga, A. Chodkowska, E. Jagiełło-Wójtowicz, K. Stępniak, A.E. Kozioł, Acta Pol. Pharm. Drug Res. 59 (2002) 281–290.

    CAS  Google Scholar 

  3. K. Walczak, A. Gondela, J. Suwiński, Eur. J. Med. Chem. 39 (2004) 849–853.

    Article  CAS  PubMed  Google Scholar 

  4. V. Klimešová, L. Zahajská, K. Waisser, J. Kaustová, U. Möllmann, Il Farmaco 59 (2004) 279–288.

    Article  PubMed  Google Scholar 

  5. K. Dabak, Ö. Sezer, A. Akar, O. Anaç, Eur. J. Med. Chem. 38 (2003) 215–218.

    Article  CAS  PubMed  Google Scholar 

  6. K. Zamanii, K. Faghihii, T. Tofighii, M.R. Shariatzadeh, Turk. J. Chem. 28 (2004) 95–100.

    Google Scholar 

  7. M. Tatarczak, J. Flieger, H. Szumiło, Chromatographia 61 (2005) 307–309.

    Article  CAS  Google Scholar 

  8. T. Tuzimski, Chromatographia 56 (2002) 379–381.

    Article  CAS  Google Scholar 

  9. L.R. Snyder, J.J. Kirkland, Introduction to Modern Liquid Chromatography, Wiley, New York, 1974.

    Google Scholar 

  10. H. Schlitt, F. Geiss, J. Chromatogr. 67 (1972) 261–266.

    Article  CAS  Google Scholar 

  11. I. Kerimov, G Ayhan-Kilcigil, B. Can-Eke, N. Altanlar, M. Iscan, J. Enzym. Inhib. Med. Chem. 22 (2007) 696–701.

    Article  CAS  Google Scholar 

  12. U. Salgın-Gökşen, N. Gökhan-Kelekçi, Ö. Göktaş, Y. Köysal, E. Kılıç, Ş. Işık, G Aktay, M. Özalp, Bioorg. Med. Chem. 15 (2007) 5738–5751.

    Article  PubMed  Google Scholar 

  13. G Küçükgüzel, A. Kocatepe, E. De Clercq, F Şahin, M. Güllüce, Eur. J. Med. Chem. 41 (2006) 353–359.

    Article  PubMed  Google Scholar 

  14. O.I. El-Sabbagh, H.M. Rady Eur. J. Med. Chem. 44 (2009) 3680–3686.

    Article  CAS  PubMed  Google Scholar 

  15. F Chan, L. Yeung, R. LoBrutto, Y. Kazakevich, J. Chromatogr. A 1082 (2005) 158–164.

    Article  CAS  PubMed  Google Scholar 

  16. S. Buntz, M. Figus, Z. Liu, Y.V. Kazakevich, J. Chromatogr. A 1240 (2012) 104–112.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Medical University, Chodźki 4a, 20-093, Lublin, Poland

    Małgorzata Tatarczak & Jolanta Flieger

  2. Department of Organic Chemistry, Medical University, Chodźki 4a, 20-093, Lublin, Poland

    Monika Wujec & Monika Pitucha

Authors
  1. Małgorzata Tatarczak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jolanta Flieger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monika Wujec
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Monika Pitucha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jolanta Flieger.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tatarczak, M., Flieger, J., Wujec, M. et al. Usefulness of Thin-Layer Chromatography for the Prediction of High-Performance Liquid Chromatographic Retention Behavior of New 1,2,4-Triazole and Thiosemicarbazide Derivatives. JPC-J Planar Chromat 28, 24–29 (2015). https://doi.org/10.1556/JPC.28.2015.1.4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1556/JPC.28.2015.1.4

Key Words

Search

Navigation