Summary
The feasibility of reversed-phase high-performance liquid chromatography for the separation of metal complexes of hematoporphyrin IX (Hp) is described. The retention order, Zn-complex<Hp (free acid)< Ni-complex<Cu-complex, is regular on an octadecylbonded stationary phase with different compositions of an aqueous methanol mobile phase. These four compounds can be successfully separated within about 8 min on a LiChrosorb RP-18 column (250×4-mm i.d.) with a 85:15 (vol/vol) mixture of methanol and phosphate buffer (pH 3) at a flow rate of 1 ml/min.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
W. I. White, R. C. Backmann, B. F. Burnharm, “The Porphyrin”,D. Dolphin, ed., Academic Press, New York, 1978; Vol.1, p. 553.
R. M. Smith, D. Doran, M. Mazur, B. Bush, J. Chromatogr.,181, 319 (1980).
V. Miller, M. Malina, J. Chromatogr.145, 290 (1978).
S. K. Hajibrahim, P. J. C. Tibbetts, C. D. Watts, J. R. Maxell, G. Eglinton, H. Colin, G. Guiochon, Anal. Chem.,50, 549 (1978).
R. H. Fish, J. J. Komlenic, Anal. Chem.,56, 519 (1984).
L. J. DeFilippi, D. E. Hultquist, Biochem. Biophys. Acta,498, 395 (1977).
W. T. Shoaf, J. Chromatogr.,152, 247 (1978).
K. Saitoh, M. Kobayashi, N. Suzuki, Anal. Chem.,53, 2309 (1981).
M. Kobayashi, K. Saitoh, N. Suzuki, Chromatographia,18, 441 (1984).
M. Kobayashi, K. Saitoh, N. Suzuki, Chromatographia,20, 49 (1985).
S. Miyake, K. Saitoh, N. Suzuki, Chromatographia,20, 417 (1985).
Y. Wakui, K. Saitoh, N. Suzuki, Chromatographia (to be published).
K. Saitoh, M. Kobayashi, N. Suzuki, J. Chromatogr.,243, 291 (1982).
M. Kobayashi, K. Saitoh, N. Suzuki, Chromatographia,20, 72 (1985).
N. Suzuki, T. Takeda, K. Saitoh, Chromatographia (to be published).
T. J. Dougherty, J. E. Kaufman, A. Goldfarb, K. R. Weishaupt, D. Boyl, A. Mittelman, Cancer Res.,38, 2628 (1978).
A. Dahlman, A. G. Wile, R. G. Burns, G. R. Mason, F. M. Johnson, M. W. Berns, Cancer Res.,43, 430 (1983).
D. Kessel, M.-L. Cheng, Cancer Res.,45, 3053 (1985).
K. Saitoh, Y. Sugiyama, N. Suzuki, J. Chromatogr. (in Press).
N. Suzuki, K. Saitoh, Y. Sugiyama, Chromatographia (submitted).
K. Saitoh, N. Suzuki, Anal. Chem.,51, 1683 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Suzuki, N., Saitoh, K. & Sugiyama, Y. High-performance liquid chromatographic separation of hematoporphyrin and its nickel, copper and zinc complexes on octadecyl-bonded stationary phase. Chromatographia 22, 132–134 (1986). https://doi.org/10.1007/BF02257313
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02257313