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Skin mast cell releasability in dogs with atopic dermtitis

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Abstract

Isolated dermal mast cells from atopic dogs are a valuable tool for the analysis of their functional properties in atopic dermatitis. We have characterized the histamine secretory pattern of mast cells enzymatically dispersed from the skin of dogs naturally suffering from this condition. The total histamine content found per isolated skin mast cell was higher in the allergic dogs than in nonatopic (control) animals (8.7 pg/mast cell versus 5.2 pg/mast cell). This phenomenon together with the well known higher concentration of skin mast cell number in atopic dermatitis lesions might account for the observed increase in local histamine concentration (15.0 μg/g versus 9.0 μg/g). Atopic dog-derived mast cells were highly reactive to both non-immunological (ionophore A23187) and an immunological-like (concanavalin A) stimulus. Furthermore, histamine net release induced by concanavalin A (1 mg/ml) stimulation was clearly enhanced in the atopic dogs (33.3% net release versus 15.4% in controls). These results have not been described in dermal mast cells dispersed from the skin of individuals with atopic dermatitis and clearly support the hypothesis that mast cells play a major role in causing and possibly modulating atopic dermatitis, through enhanced sensitivity or releasability. however, whether these two phenomena are primary abnormalities of atopic dermatitis, or only secondary changes, remains undetermined.

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References

  1. Willemse TA. Atopic dermatitis. In: Nesbitt GH, editor. Contemporary issues in small animal practice. Dermatology volume. Edinburgh: Churchill Livingstone, 1987:57–77.

    Google Scholar 

  2. Rhodes KH, Kerdel F, Soter N. Comparative aspects of canine and human atopic dermatitis. Sem Vet Derm Surg 1987;2:166–72.

    Google Scholar 

  3. Schwartzman RM, Massicot JG, Sogn DD, Cohen SG. The atopic dog model: report of an attempt to establish a colony. Int Arch Allergy Appl Immunol 1983;72:97–101.

    PubMed  Google Scholar 

  4. Butler JM, Peters JE, Hirshman CA, White CR, Margolin LB, Hanifin JM. Pruritic dermatitis in asthmatic basenji-greyhound dogs: a model for human atopic dermatitis. J Am Acad Dermatol 1983;8:33–8.

    PubMed  Google Scholar 

  5. Holgate ST, Church MK. The mast cell. Br Med Bull 1992; 48:40–50.

    PubMed  Google Scholar 

  6. Sampson HA. Role of immediate hypersensitivity in the pathogenesis of atopic dermatitis. Allergy 1989;44 Suppl 9: 52–8.

    Google Scholar 

  7. Rothe MJ, Nowak M, Kerdel FA. The mast cell in health and disease. J Am Acad Dermatol 1990;23:615–24.

    PubMed  Google Scholar 

  8. Becker AB, Fan Chung K, McDonald DM, Lazarus SC, Frick OL, Gold WM. Cutaneous mast cell heterogeneity: response to antigen in atopic dogs. J All Clin Immunol 1986;78:937–42.

    Article  Google Scholar 

  9. Holgate ST. The mast cell and its function in allergic disease. Clin Exp Allergy 1991;21 Suppl 3:11–6.

    PubMed  Google Scholar 

  10. Hanifin JM. Immunologic aspects of atopic dermatitis. Dermatol Clin 1990;8:747–50.

    PubMed  Google Scholar 

  11. Nimmo Wilkie JS, Yager JA, Eyre P, Parker WM. Morphometric analyses of the skin of dogs with atopic dermatitis and correlations with cutaneous and plasma histamine and total serum IgE. Vet Pathol 1990;27:179–86.

    PubMed  Google Scholar 

  12. Mihm MC, Soter NA, Dvorak HF, Austen KF. The structure of normal skin and the morphology of atopic eczema. J Invest Dermatol 1976;67:305–12.

    Article  PubMed  Google Scholar 

  13. Irani AM, Sampson HA, Schwartz LB. Mast cells in atopic dermatitis. Allergy 1989;44 Suppl 9:31–4.

    PubMed  Google Scholar 

  14. Johnson HH, DeOreo GA, Lasheld WP, Mitchell F. Skin histamine levels in chronic atopic dermatitis. J Invest Dermatol 1960;34:237–8.

    PubMed  Google Scholar 

  15. Ruzicka T, Glück S. Cutaneous histamine levels and histamine releasability from the skin in atopic dermatitis and hyper-IgEsyndrome. Arch Dermatol Res 1983;275:41–4.

    Article  PubMed  Google Scholar 

  16. Juhlin L. Localization and content of histamine in normal and diseased skin. Acta Derm Venereol (Stockh) 1967;47:383–91.

    Google Scholar 

  17. Flint KC, Leung KBP, Pearce FL, Hudspith BN, Brostoff J, Johnson NM. Human mast cells recovered by bronchoalveolar lavage: their morphology, histamine release and the effects of sodium cromoglycate. Clin Sci 1985;68:427–32.

    PubMed  Google Scholar 

  18. Walls AF, Djukanovic R, Walters C, Wilson JW, Britten KM, Wilson SJ et al. Mast cell activation in atopic and intrinsic asthma. J Allergy Clin Immunol 1991;87:215.

    Article  Google Scholar 

  19. Hirshman CA, Austin DR, Kettelkamp NS. Enhanced bronchoalveolar lavage mast cells histamine releasability in allergic dogs with and without airway hyperresponsiveness. J All clin Immunol 1988;81:829–35.

    Article  Google Scholar 

  20. Turner CR, Darowski MJ, Sampson HA, Spannhake EW, Hirshman CA. Dermal mast cell releasability and end organ responsiveness in atopic and nonatropic dogs. J All Clin Immunol 1989;83:643–8.

    Article  Google Scholar 

  21. Lowman MA, Rees PH, Benyon RC, Church MK. Human mast cell heterogeneity: histamine release from mast cells dispersed from skin, lung, adenoids, tonsils and intestinal mucosa in response to IgE-dependent and non-immunological stimuli. J All Clin Immunol 1988;81:590–7.

    Article  Google Scholar 

  22. Robinson C, Benyon C, Holgate ST, Church MK, The IgE-and calcium-dependent release of eicosanoids and histamine from human purified cutaneous mast cells. J Invest Dermatol 1989;93:397–404.

    Article  Google Scholar 

  23. Okayama Y, Church MK. Comparison of the modulatory effect of ketotifen, sodium cromoglycate, procaterol and salbutamol in human skin, lung and tonsil mast cells. Int Arch All Immunol 1992;97:216–25.

    Google Scholar 

  24. de Mora F, García G, Ferrer L, Arboix M. Canine cutaneous mast cells dispersion and histamine secretory characterization. Vet Immunol Immunopathol 1993;39:421–9.

    Article  PubMed  Google Scholar 

  25. Benyon RC, Church MK, Clegg LS, Holgate ST. Dispersion and characterisation of mast cells from human skin. Int Arch All Appl Immunol 1986;79:332–4.

    Google Scholar 

  26. Kimura I, Moritani Y, Tanizaki Y, Basophils in bronchial asthma with reference to reagin-type allergy. Clin Allergy 1973;3:195–202.

    PubMed  Google Scholar 

  27. Shore PA, Burkhalter A, Cohn VH. A method for the fluorometric assay of histamine in tissues. J Pharm Exp Ther 1959;127:182–6.

    Google Scholar 

  28. Bergendorff A, Uvnäs B. Storage of 5-hydroxytryptamine in rat mast cells. Evidence for an ionic binding to carboxyl groups in a granule heparin-protein complex. Acta Physiol Scand 1972;84:320–31.

    PubMed  Google Scholar 

  29. Hirata F, Axelrod J, Crews FT. Concanavalin A stimulates phospholipid methylation and phosphatidylserine decarboxilation in rat mast cells. Proc Natl Acad Sci USA 1979;76:4813–6

    Google Scholar 

  30. Lichtenstein LM, MacGlashan DW. The concept of basophil releasability. J All Clin Immunol 1986;77:291–4.

    Google Scholar 

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Authors and Affiliations

  1. Unitat de Farmacologia, Facultat de Veterinària, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Spain

    F. DeMora, G. García, A. Puigdemont & M. Arboix

  2. Department de Patologia i Produccció Animals, Universitat Autòmama de Barcelona, E-08193, Bellaterra, Barcelona, Spain

    L. Ferrer

Authors
  1. F. DeMora
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  2. G. García
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  3. A. Puigdemont
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  4. M. Arboix
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  5. L. Ferrer
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accepted by W. Lorenz

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DeMora, F., García, G., Puigdemont, A. et al. Skin mast cell releasability in dogs with atopic dermtitis. Inflamm Res 45, 424–427 (1996). https://doi.org/10.1007/BF02252939

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  • DOI: https://doi.org/10.1007/BF02252939

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