Summary
The first 30-min. gastric juice specimen following augmented histamine stimulation was collected from 32 patients. After measuring the volume and pH, each sample was divided into 2 equal parts, and one half was neutralized to pH 7.0. The paired samples were individually dialyzed and lyophilized, and their weight, hexose and nitrogen content, proteolytic enzyme activity, and B12 binding capacity were determined. When the paired samples were compared, in-vitro neutralization and dialysis reduced the concentration of the proteolytic enzyme activity and increased the concentration of hexoses and B12binding materials. In-vitro neutralization and dialysis reduced the weight of the samples and the total proteolytic enzyme activity, while the total B12 binding material was increased. The nitrogen concentration was reduced also by in-vitro neutralization and dialysis.
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Supported in part by Training Grant TI AM-5179 and by Grants AM 04775 and AM 06298 from the National Institutes of Health, U. S. Public Health Service.
The authors acknowledge the assistance of the Biostatistical Unit and Medical Research Computer Center of The University of Oklahoma Medical Center in the data analysis.
The Co60B12 used in this study was kindly supplied through the assistance of Gerald L. Beckloff, M.D., Squibb Institute for Medical Research, New Brunswick, N. J.
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Welsh, J.D., Wallace, C.D., Walker, A. et al. Effects of in-vitro neutralization on the composition of the nondialyzable components of human gastric juice. Digest Dis Sci 12, 389–395 (1967). https://doi.org/10.1007/BF02241941
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DOI: https://doi.org/10.1007/BF02241941