Skip to main content
SpringerLink
Log in

Gel filtration of Vitamin B12 binders and intrinsic factor from human gastric juice on sephadex columns

  • Published:
The American Journal of Digestive Diseases Aims and scope Submit manuscript

Summary and Conclusions

Normal gastric juice-bound Vitamin B12 was excluded from all Sephadex columns, including G-200. Fractions excluded from the G-75, G-100, and G-200 column, exhibited intrinsic-factor activity on urinary excretion test.

Since materials excluded from Sephadex G-200 supposedly have a molecular weight of over 200,000, one may assume that the intrinsic-factor-related B12 binders, as well as intrinsic factor itself, have a molecular weight in this high range, or that they polymerize or form a complex with other macromolecular gastric materials of this size range.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gräsbeck, R. Studies on the vitamin B12 binding principle and other biocolloids of human gastric juice.Acta med. scandinav. (Suppl.)314:87, 1956.

    Google Scholar 

  2. Glass, G. B. J. Localization of intrinsic factor and the sites of B12 binding in the paper electrophoresis of human gastric juices and concentrates from hog gastric mucosa.Haematol. latina (Milan) 2:231, 1959.

    Google Scholar 

  3. Glass, G. B. J., andUchino, H. Vitamin B12 binders in human gastric juice (abstr).Fed. Proc. 18:53, 1959.

    Google Scholar 

  4. Gullberg, R. Electrophoretic fractionation of B12 binders in gastric juices from patients with pernicious anemia and from controls.Proc. Soc. Exper. Biol. & Med. 105:62, 1960.

    Google Scholar 

  5. Glass, G. B. J., Uchino, H., and Schwartz, G. Study of vitamin B12 binders in the gastric juice of normals, of patients with pernicious anemia and of those with atrophic gastritis and histamine-fast anacidity.Proc. VIII Internat. Congress Hematol., Tokyo, Sept. 4–10, 1960. Pan-Pacific Press, Tokyo,2:956, 1962.

    Google Scholar 

  6. Jeffries, G. J., Smith, F. W., Hoskins, S. W., andSleisenger, M. H. Fractionation of the non-dialyzable components of normal human gastric juice by starch gel electrophoresis.Gastroenterology 41:467, 1961.

    Google Scholar 

  7. Uchino, H., Schwartz, G. H., andGlass, G. B. J. Paper electrophoretic analysis of B12 binding in human gastric juice.Clin. chim. acta. 9:461, 1964.

    Google Scholar 

  8. Uchino, H., Schwartz, G. H., andGlass, G. B. J. Effects of peptic digestion, pH change and heating on B12 binders in the electrophoretic partition of the human gastric juice.Clin. chim. acta 9:474, 1964.

    Google Scholar 

  9. Kakei, M., andGlass, G. B. J. Separation of “primary” and “secondary” B12 binders in human gastric juice by paper electrophoresis at low pH.Clin. chim. acta 9:485, 1964.

    Google Scholar 

  10. Glass, G. B. J., Stephanson, L., Rich, M., andLaughton, R. W. Intrinsic-factor activity of human gastric juice after fractionation by continuous electrophoresis on paper curtain.Brit. J. Haematol. 3:401, 1957.

    Google Scholar 

  11. Gräsbeck, R., Simons, K., andSinkkonen, I. Purification of intrinsic factor and vitamin B12 binders from human gastric juice.Ann. med. exper. et biol. Fenniae (Suppl.)40:6, 1962.

    Google Scholar 

  12. Glass, G. B. J. Biologically active materials related to gastric mucus in the normal and in the diseased stomach of man.Gastroenterology 43:310, 1962.

    Google Scholar 

  13. Glass, G. B. J., Kakei, M., Kubo, K., andStephanson-Liounis, L. Fractionation of mucosubstances and proteins in human gastric juice by gel filtration on Sephadex columns combined with paper electrophoresis.Proc. VII Internat. Congress Gastroenterol., Brussels. 2:158, 1964.

    Google Scholar 

  14. Kakei, M., andGlass, G. B. J. Separation of bound and free vitamin B12 on Sephadex G-25 column (abstr).Fed. Proc. 21:469, 1962; Proc. Soc. Exper. Biol. & Med. 111:270, 1962.

    Google Scholar 

  15. Glass, G. B. J., Kakei, M., Kubo, K., Lee, D. H., andHorton, T. Separation of intrinsic factor and vit. B12 binders from human gastric juice by gel filtration on Sephadex (abstr.).Fed. Proc. 22: 204, 1963.

    Google Scholar 

  16. Porath, J., andFlodin, P. Gel filtration: A method for desalting and group separation.Nature 183:1657, 1959.

    Google Scholar 

  17. Flodin, P. Dextran gels and their applications in gel filtration. Dissertation, Uppsala, Sweden, 1962.

    Google Scholar 

  18. Ishimori, A., andGlass, G. B. J. Paper electrophoresis of native gastric juice after concentration by dialysis against Carbowax 6000.Clin. Chem. 7: 457, 1961.

    Google Scholar 

  19. Glass, G. B. J., Stephanson, L., andRich, M. Paper electrophoretic analysis of gastric juice in health and disease and its physiological and clinical significance.Gastroenterologia 86:384, 1956.

    Google Scholar 

  20. Glass, G. B. J. Paper electrophoresis of gastric juice in health and disease.Am. J. Digest. Dis. 6: 1131, 1961.

    Google Scholar 

  21. Schilling, R. F. Intrinsic factor studies. II. The effect of gastric juice on the urinary excretion of radioactivity after the oral administration of radioactive vitamin B12.J. Lab. & Clin. Med. 42:860, 1953.

    Google Scholar 

  22. Glass, G. B. J. Gastric intrinsic factor and its function in the metabolism of vitamin B12.Physiol. Rev. 43:529–849, 1963.

    Google Scholar 

  23. Yamaguchi, K., and Glass, G. B. J. Unpublished data.

Download references

Author information

Authors and Affiliations

  1. From the Gastroenterology Research Laboratory and Section of Gastroenterology, Department of Medicine, New York Medical College-Metropolitan Hospital Medical Center, New York, N. Y.

    Katsuhiko Kubo M.D., Mamoru Kakei M.D., George B. Jerzy Glass M.D. & Marion Dolinski M.S.

Authors
  1. Katsuhiko Kubo M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mamoru Kakei M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. George B. Jerzy Glass M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marion Dolinski M.S.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by Research Grant-in-Aid AM-00068 (12) from the National Institute of Arthritis and Metabolic Diseases, and the American Cancer Society [P-305 (F)].

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kubo, K., Kakei, M., Glass, G.B.J. et al. Gel filtration of Vitamin B12 binders and intrinsic factor from human gastric juice on sephadex columns. Digest Dis Sci 10, 355–366 (1965). https://doi.org/10.1007/BF02233427

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02233427

Keywords

Search

Navigation