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Hepatic uptake and blood clearance of free Co57B12 and of Co57B12 bound to gastric juice after intravenous injection in man

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Summary

In 9 normal subjects, hepatic uptake and blood clearance of radioactivity were measured for 7–10 days after the intravenous administration of a tracer dose of Co57B12 alone. In 5 of these subjects, the measurements were repeated after intravenous administration of Co57B12 bound to normal human gastric juice (NHGJ). Administration of B12 bound to NHGJ resulted in an augmented hepatic uptake of the vitamin at 1 hr. significantly greater than that for B12 alone. This difference became less marked with time and was not significant at the end of 1 week. Blood clearance during the first 24 hr. following administration was significantly slower for NHGJ-bound B12 than for B12 injected alone.

These and other findings from our laboratory suggest that IF-related gastric B12 binders and some non-IF-related binders may function in the hepatic uptake of vitamin B12, if administered intravenously in man.

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Author notes

  1. Herbert Weisberg M.D.

    Present address: U. S. Public Health Service Trainee in Gastroenterology, USA

Authors and Affiliations

  1. From the Section of Gastroenterology, Department of Medicine, New York Medical College-Metropolitan Hospital Medical Center, New York, N. Y.

    William S. Rosenthal M.D., Herbert Weisberg M.D., George B. Jerzy Glass M.D. & William Priest

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  1. William S. Rosenthal M.D.
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  2. Herbert Weisberg M.D.
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  3. George B. Jerzy Glass M.D.
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  4. William Priest
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Additional information

Supported in part by Research Grant-in-Aid AM-00068-11 and 12, Training Grant TI-AM-5237, and Clinical Research Center Grant AM-05576-02 of the National Institute of Arthritis and Metabolic Diseases, N.I.H., U.S. Public Health Service, and Contract U-1373 of the Health Research Council of the City of New York.

We gratefully acknowledge the assistance of Mrs. Carrie Gunnis. Radioactive Vitamin B12 used in the study was generously supplied by Dr. Elmer Alpert and Dr. Charles Rosenblum of Merck Sharp & Dohme Research Laboratories, West Point, Pa.

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Rosenthal, W.S., Weisberg, H., Glass, G.B.J. et al. Hepatic uptake and blood clearance of free Co57B12 and of Co57B12 bound to gastric juice after intravenous injection in man. Digest Dis Sci 10, 346–354 (1965). https://doi.org/10.1007/BF02233426

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