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Regulation of IGF binding proteins by FSH in human luteinizing granulosa cells

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Abstract

Objectives

To evaluate the regulation of the secretion of insulin-like growth factor (IGF) binding proteins (IGFBPs) by FSH in human luteinizing granulosa cells.

Design and Results

Luteinizing granulosa cells were incubated with and without FSH. Levels of IGFBP-1 and -3 in the medium were measured by EIA and RIA, respectively and binding activities of these IGFBP were evaluated by Western ligand blot. FSH inhibited the secretion of IGFBP-1 dose dependently. FSH did not inhibit the secretion of immunoreactive IGFBPS, but inhibited the binding activity of IGFBP-3. To assess the protease activity,125 I-IGFBP-3 was incubated with the cultured medium.125 I-IGFBP-3 degraded into small fragments when it was incubated with the cultured medium treated with FSH.

Conclusions

FSH enhances the action of IGF-I in human granulosa cells by inhibiting the secretion of IGFBP-1 and the binding activity of IGFBP-3 by stimulating the proteolysis of IGFBPS-3.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Tokyo Women's Medical College, 8-1 Kawada-cho Shinjuku-ku, 162, Tokyo, Japan

    Tomoko Adachi, Mitsutoshi Iwashita & Yoshihiko Takeda

  2. Department of Obstetrics and Gynecology, Tokyo Women's Medical College Daini Hospital, 2-1-10 Nishiogu Arakawa-ku, 116, Tokyo, Japan

    Atsuko Kuroshima

Authors
  1. Tomoko Adachi
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  2. Mitsutoshi Iwashita
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  3. Atsuko Kuroshima
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  4. Yoshihiko Takeda
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Adachi, T., Iwashita, M., Kuroshima, A. et al. Regulation of IGF binding proteins by FSH in human luteinizing granulosa cells. J Assist Reprod Genet 12, 639–643 (1995). https://doi.org/10.1007/BF02212589

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  • DOI: https://doi.org/10.1007/BF02212589

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