Summary
Lipopolysaccharides (LPS) were extracted from two strains ofRhizobium japonicum (61A76NS and 3I1b110-I). The extracted LPS was purified by gel filtration column chromatography and the amount of 2-keto-3-deoxyoctonate (KDO) was determined. Column purified LPS from both strains were conjugated to rhodamine isothiocyanate on celite to examine binding of this purified, labeled surface component to aseptically grownGlycine soja (wild soybean) seedlings as a basis for symbiotic specificity using fluorescent microscopy. Rhodamine conjugated LPS from both strains ofRhizobium japonicum did not exhibit specific binding to wild soybean seedling roots.
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References
Ashwell G 1966 The phenol-sulfuric acid reaction for carbohydrates. Meth. Enzymol. 3, 93–94.
Bohlool B and Schmidt E 1974 Lectins: Possible basis for specificity in the Rhizobiumlegume root nodule symbiosis. Science 185, 269–271.
Calvert H, Lalonde E M, Bhuvoneswari T B and Bauer W D 1978 Role of lectins in plant micro-organism interactions. IV. Ultrastructure localization of soybean lectin binding sites onRhizobium japonicum. Can. J. Microbiol. 24, 785–792.
Cole M A and Elkan G H 1973 Transmissible resistance to penicillin G, neomycin, and chloramphenicol inRhizobium japonicum. Antimicrob. Agents Chemother. 4, 248–253.
Dazzo F B and Brill W 1976 Receptor sites on clover and alfalfa roots for Rhizobium. Appl. Environ. Microbiol. 33, 132–136.
Debelder A N and Granath R 1973 Preparation and properties of fluorscein labelled dextrans. Carbo. Res. 30, 375–378.
Hughes T A, Leece J G and Elkan G H 1979 Modified fluorescent technique, using rhodamine for studies ofRhizobium japonicum-soybean symbiosis. Appl. Environ. Microbiol. 37, 1243–1244.
Kamberger W 1979 An oughterlony double diffusion study on the interaction between legume lectins and rhizobial cell surface antigens. Arch. Microbiol. 121, 83–90.
Karkanis Y D, Zeltner J Y, Jackson J J and Carlo D J 1977 A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharides of gram negative bacteria. Anal. Biochem. 85, 595–601.
Westphal O and Jann K 1965 Bacterial lipopolysaccharides, extraction with phenol-water and further applications of the procedure. Meth. Carbo. Chem. 5, 83–91.
Wolpert J S and Albersheim P 1976 Host-symbiont interactions. I. The lectins of legumes interact with the 0-antigen-containing lipopolysaccharides of their symbiont Rhizobia. Biochem. Biophys. Res. Comm. 70, 729–737.
Zevenhuizen L P T M and Scholten-Koerselman H J 1979 Surface carbohydrates of Rhizobium. Antonie Van Leeuwenhoek. 45, 165–175.
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Paper no. 8130 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27650, USA.
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Hughes, T.A., Elkan, G.H. Lack of specific binding of labeledRhizobium japonicum lipopolysaccharides to wild soybean (Glycine soja) roots. Plant Soil 84, 115–119 (1985). https://doi.org/10.1007/BF02197872
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DOI: https://doi.org/10.1007/BF02197872