Abstract
Nod factors (NF) are lipochitooligosaccharides produced by nitrogen-fixing rhizobia bacteria. They are key components of the rhizobia-plant signaling exchange required for symbiosis. Thus, techniques to extract, detect, characterize, and purify NF are crucial for the identification of both rhizobial and plant mechanisms underlying nitrogen-fixing symbiosis. Here, we describe a method for NF detection using radiolabeling and thin-layer chromatography. Furthermore, we describe a technique for purifying NF for downstream analyses.
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Jacott, C.N., Lozano-Morillo, S., del Cerro, P. (2024). Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes. In: Medina, C., López-Baena, F.J. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 2751. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3617-6_16
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DOI: https://doi.org/10.1007/978-1-0716-3617-6_16
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