Abstract
The blood samples from fifteen normal lactating buffaloes were taken from December 15th 1978 to 31st August, 1979. Depending upon the climatic conditions, the whole period of study was divided into four seasons. The mean values of carbonic anhydrase (moles CO2/l/sec×10−5) were 3.08±0.26, 4.94±0.44, 5.23±0.35, 6.44±0.32 in pregnant and 4.87±0.27, 4.53±0.41, 4.74±0.45, 6.36±0.40 in non-pregnant animals during winter, spring, hot and dry and hot and humid seasons. Mean values of pO2 (mm Hg) were 31.26±1.41, 31.92±0.61, 35.90±0.59, 33.80 ±0.67 in pregnant and 31.89±0.44, 31.53±0.54, 35.52±0.69, 31.65±0.95 in non-pregnant buffaloes during winter, spring, hot and dry and hot and humid periods, respectively. There were highly significant (P< 0.01) differences between seasons with respect to pO2, pCO2, actual HCO3 and heamoglobin. However, PCV changed significantly (P<0.01) with the physiological status of the animal. Different correlation of biochemical parameters with climatic elements were discussed. Thus, the shifts in the levels of carbonic anhydrase, HCO3 and heamoglobin may prove to be a better tool/index for thermal stress in buffaloes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
ASSALI, N. S. (1968):Biology of Gestation. Academic Press, N.Y.
GABRIEL, B. and PINCUS, G. (1962): Effect of estrogen and progesterone on uterine carbonic anhydrase of immature rats. Endocrinology, 70: 781–785.
GAMBINO, S. R. (1961): Collection of capillary blood for simultaneous determination of arterial pH, CO2 and oxygen saturation. Am. J. Clin. Path., 30: 175–180.
HARVEY, W. R. (1966): Least squares analysis of data with unequal sub-class numbers. United States, Department of Agriculture. ARS. 20-B.
KEELE, C. A. and NEIL, E. (1974):Sampson Wright's Applied Physiology. 11th ed. The English Language Book Society and Oxford University Press.
MAREN, T. H. (1967): Carbonic anhydrase: Chemistry, physiology and inhibition. Physiol. Rev. 47: 597–781.
PANDEY, M. D. and ROY, A. (1969): Studies on the adaptability of buffaloes to tropical climate: Seasonal changes in the water and electrolyte status of buffalo cow. Indian J. Anim. Sci., 39: 367–377.
ROUGHTON, F. J. W. and BOOTH, V. H. (1946): Determination of carbonic anhydrase. Biochem., 40: 319–340.
ROUGHTON, F. J. W. (1954): InHandbook of Respiratory Physiology 2nd ed. U.S.A.E. School of Aviation Medicine, Randolph AFB, Texas.
SHARMA, D. D. and CHANDER, S. (1970): Observation on the blood haemoglobin level in cattle and buffaloes under field conditions in temperate zone. Ind. J. Dairy Sci. 23: 85–90.
SIGGARD-ANDERSON, O. (1970): Blood acid base alignment nomograph, Scand. J. Clin. Lab. Invest. Radiometer reprint. AS-21.
SNEDECOR, G. W. and COCHRAN, W. G. (1967):Statistical Methods. 6th Ed. Calcutta. Oxford and IBH.
SWENSON, M. J. (1970):Duke's Physiology of Domestic Animals. 8th ed. Comstock Publishing Associates, Cornell University Press.
WONG, J. (1928): The determination of iron and haemoglobin in blood. J. Biol. Chem., 77: 409–418.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mehta, S.N., Gangwar, P.C. Carbonic anhydrase and acid base balance in relation to thermal stress in buffaloes (Bubalus bubalis). Int J Biometeorol 27, 41–46 (1983). https://doi.org/10.1007/BF02186299
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02186299