Summary
The optimum conditions for growth ofFrankia sp. HFPCcI3 isolated fromCasuarina cunninghamiana, were studied in batch culture using defined media. Maximum growth (doubling time was 24 h)_was achieved at 33°C and at pH 6.3 with pyruvate and NH +4 as the sole C and N sources, respectively. Removal of NH +4 from the culture medium resulted in vesicle differentiation which was paralleled by induction of acetylene reduction activity. Growth on atmospheric N2 was optimal with combined pyruvate and glucose as the carbon sources and displayed a doubling time of about 48 h. Comparisons in growth and N2-fixing activity ofFrankia strains grown in a variety of cultural conditions demonstrate the range of behavior among the strains.
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Zhongze, Z., Murry, M.A. & Torrey, J.G. Culture conditions influencing growth and nitrogen fixation inFrankia sp.HFPCcI3 isolated from Casuarina. Plant Soil 91, 3–15 (1986). https://doi.org/10.1007/BF02181814
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DOI: https://doi.org/10.1007/BF02181814