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Molecular characterization of the lysosomal acid phosphatase fromDrosophila melanogaster

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Abstract

InDrosophila, unlike humans, the lysosomal acid phosphatase (Acph-1) is a non-essential enzyme. It is also one of the most rapidly evolving gene-enzyme systems in the genus. In order to determine which parts of the enzyme are conserved and which parts are apparently under little functional constraint, we cloned the gene fromDrosophila melanogaster via a chromosomal walk. Fragments from the gene were used to recover an apparently full-length cDNA. The cDNA was subcloned into aDrosophila transformation vector where it was under the control of the 5′ promoter sequence of thehsp-70 gene. Three independent transformants were obtained; in each, Acph-1 expression from the cDNA was constitutive and not dependent on heat shock, as determined by densitometric analyses of the allozymic forms of the enzyme. The pattern of expression indicates thehsp-70 and endogenousAcph-1 promoters act together in some, but not all, tissues. The sequence of the cDNA was determined using deletions made with exonuclease III, and primers deduced from the cDNA sequence were used to sequence the genomic clone. Five introns were found, and putative 5′ up-stream regulatory sequences were identified. Amino acid sequence comparisons have revealed several highly conserved motifs betweenDrosophila Acph-1 and vertebrate lysosomal and prostatic acid phosphatases.

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Authors and Affiliations

  1. Section of Genetics and Development, Cornell University, 101 Biotechnology Building, 14853, Ithaca, NY, USA

    Ross MacIntyre

  2. Department of Environmental Engineering, Dongshin University, Korea

    Hae-Jin Chung

  3. Washington University, 152 McDonnell Hall, 63130, St. Louis, MO, USA

    Christopher Shaffer

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  1. Hae-Jin Chung
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  2. Christopher Shaffer
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  3. Ross MacIntyre
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Communicated by M. Ashburner

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Chung, HJ., Shaffer, C. & MacIntyre, R. Molecular characterization of the lysosomal acid phosphatase fromDrosophila melanogaster . Molec. Gen. Genet. 250, 635–646 (1996). https://doi.org/10.1007/BF02174451

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