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Nucleotide sequence and expression of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA) fromAgaricus bisporus

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Abstract

The gene encoding NADP+-dependent glutamate dehydrogenase (gdhA) was isolated from anAgaricus bisporus recombinant phageλ library. The deduced amino acid sequence would specify a 457-amino acid protein that is highly homologous in sequence to those derived from previously isolated and characterized genes coding for microbial NADP+-GDH. The open reading frame is interrupted by six introns. None of the introns is located at either one of the positions of the two introns conserved in the corresponding open reading frames of the ascomycete fungiAspergillus nidulans andNeurospora crassa. Northern analysis suggests that theA. bisporus gdhA gene is transcriptionally regulated and that, unlike the case in ascomycetes, transcription of this gene is repressed upon the addition of ammonium to the culture.

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References

  • Ahmad I, Hellebust JA (1991) Enzymology of nitrogen assimilation in mycorrhiza. In: Norris JR, Read DJ, Varma AK (eds) Methods in microbiology, vol. 23. Academic Press, London, pp 181–202

    Google Scholar 

  • Baars JJP, Op den Camp HJM, Hermans JMH, Mikes V, Van der Drift C, Van Griensven LJLD, Vogels GD (1994) Nitrogen assimilating enzymes in the white button mushroomAgaricus bisporus. Microbiology 140:1161–1168

    Google Scholar 

  • Baars JJP, Op den Camp HJM, Van Hoek AHAM, Van der Drift C, Van Griensven LJLD, Visser J, Vogels GD (1995a) Purification and characterization of NADP-dependent glutamate dehydrogenase from the commercial mushroomAgaricus bisporus. Curr Microbiol 30:211–217

    Google Scholar 

  • Baars JJP, Op den Camp HJM, Paalman JWG, Mikeš V, Van der Drift C, Van Griensven LJLD, Vogels GD (1995b) Purification and characterization of glutamine synthetase from the commercial mushroomAgaricus bisporus. Curr Microbiol 31:108–113

    Google Scholar 

  • Calzone FJ, Britten RJ, Davidson EH (1987) Mapping of gene transcripts by nuclease protection assays and cDNA primer extension. Methods Enzymol 152:611–632

    Google Scholar 

  • De Graaff LH, Van den Broek H, Visser J (1988) Isolation and expression of theAspergillus nidulans pyruvate kinase gene. Curr Genet 11:499–503

    Google Scholar 

  • De Groot PWJ, Van Griensven LJLD, Schaap PJ, Visser J (1995) Isolation and characterization of genes involved in growth and maintenance of fruit bodies ofAgaricus bisporus. In: Elliott EJ (ed) Science and cultivation of edible fungi. Balkema, Rotterdam, pp 83–86

    Google Scholar 

  • Dennen DW, Niederpruem DJ (1964) Control of glutamate dehydrogenase in the basidiomyceteSchizophyllum commune. Life Sci 4:93–98

    Google Scholar 

  • Dunn-Colemann NS, Tomsett AB, Garrett RH (1979) Nitrogen metabolite repression of nitrate reductase inNeurospora crassa. Effect of thegln-1a locus. J Bacteriol 139:697–700

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restiction fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Harmsen MC, Schuren FHJ, Moukha SM, Van Zuilen CM, Punt PJ, Wessels JGH (1992) Sequence analysis of glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetesSchizophyllum commune, Phanerochaete chrysosporium andAgaricus bisporus. Curr Genet 22:447–454

    Google Scholar 

  • Hawkins AR, Gurr SJ, Montague P, Kinghorn J (1989) Nucleotide sequence and regulation of expression of theAspergillus nidulans gdhA gene encoding NADP-dependent glutamate dehydrogenase. Mol Gen Genet 218:105–111

    Google Scholar 

  • Humphrey T, Proudfoot NJ (1988) A beginning to the biochemistry of polyadenylation. Trends Genet 4:243–245

    Google Scholar 

  • Jennings DH (1988) Some perspectives on nitrogen and phosporus metabolism in fungi. In: Boddy L, Marchant R, Read DJ (eds) Nitrogen, phosphorus and sulphur utilization by fungi. Cambridge University Press, Cambridge, pp 1–31

    Google Scholar 

  • Kanamori K, Legerton T, Weis RL, Roberts JD (1982) Effect of the nitrogen source on glutamine and alanine biosynthesis inNeurospora crassa. J Biol Chem 257:14168–14172

    Google Scholar 

  • Kinghorn JR, Pateman JA (1973) NAD and NADPl-glutamate dehydrogenase activity and ammonium regulation inAspergillus nidulans. J Gen Microbiol 78:39–46

    Google Scholar 

  • Kinnaird JH, Fincham JRS (1983) The complete nucleotide sequence of theNeurospora crassa am (NADP-specific glutamate dehydrogenase) gene. Gene 26:253–260

    Google Scholar 

  • Kozak M (1989) The scanning model for translation: an update. J Cell Biol 108:229–241

    Google Scholar 

  • Kusnan MB, Klug K, Fock HP (1989) Ammonia assimilation byAspergillus nidulans;15N ammonia study. J Gen Microbiol 78:39–46

    Google Scholar 

  • Lea PJ, Joy KW, Ramos JL, Guerrero MG (1984) The action of 2 amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants. Phytochemistry 23:1–6

    Google Scholar 

  • Martin F, Steward GR, Genetet I, Mourot B (1988) The involvement of glutamate dehydrogenase and glutamine synthetase in ammonia assimilation by the rapidly growing ectomycorrhizal ascomycete,Cenococcum geophilum. New Phytol 93:415–422

    Google Scholar 

  • Mattaj IW, McPherson MJ, Wootton JC (1982) Localisation of a strongly conserved section of coding sequence in glutamate dehydrogenase genes. FEBS Lett 147:21–25

    Google Scholar 

  • McPherson MJ, Wootton JC (1983) Complete nucleotide sequence of theEscherichia coli gdhA gene. Nucleic Acids Res 11:5257–5266

    Google Scholar 

  • Nagasu T, Hall BD (1985) Nucleotide sequence of theGDH gene coding for the NADP specific glutamate dehydrogenase ofSaccharomyces cerevisiae. Gene 37:247–253

    Google Scholar 

  • Nussinov R (1990) Sequence signals in eukaryotic upstream elements. Crit Rev Biochem Mol Biol 25:185–221

    Google Scholar 

  • Pateman JA (1969) Regulation of the synthesis of glutamic dehydrogenase and glutamine synthetase in micro-organisms. Biochem J 115:769–775

    Google Scholar 

  • Raper CA, Raper JR, Miller RE (1972) Genetic analysis of the life-cycle ofAgaricus bisporus. Mycologia 64:1088–1117

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schaap PJ, Müller Y, De Groot PWJ, Visser J, Baars JJP, Sonnenberg ASM, Van Griensven LJLD (1995a) Molecular characterization of genes involved in carbon metabolism and ammonium assimilation of the button mushroomAgaricus bisporus. In: Elliott TJ (ed) Science and cultivation of edible fungi. Balkema, Rotterdam, pp 103–109

    Google Scholar 

  • Schaap PJ, De Groot PWJ, Müller Y, Van Griensven LJLD, Visser J (1995b) Molecular cloning and sequence of the cytoplasmic ribosomal protein S15a gene fromAgaricus bisporus. Exp Mycol 19:160–162

    Google Scholar 

  • Schwartz T, Kusnan MB, Fock HP (1991) The involvement of glutamate dehydrogenase and glutamine synthetase/glutamate synthase in ammonium assimilation by the basidiomycete fungusStropharia semiglobata. J Gen Microbiol 137:2253–2258

    Google Scholar 

  • Scott Moye W, Amuro N, Mohana Rao JK, Zalkin H (1985) Nucleotide sequence of yeastGDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. J Biol Chem 260:8502–8508

    Google Scholar 

  • Smith EL, Austen BM, Blumenthal KM, Nye JF (1975) Glutamate dehydrogenase. In: Boyer PD (ed) The enzymes, vol XI. Academic Press, New York, pp 293–367

    Google Scholar 

  • Sonnenberg AS, Den Hollander K, Van de Munckhof APJ, Van Griensven LJLD (1991) Chromosome separation and assigment of DNA probes inAgaricus bisporus. In: Van Griensven LJLD (ed) Genetics and breeding ofAgaricus. Pudoc, Wageningen, pp 57–61

    Google Scholar 

  • Steward GR, Moore D (1974) The activities of glutamate dehydrogenases during mycelial growth and sporophore development inCoprinus lagopus (sensu Lewis). J Gen Microbiol 83:73–81

    Google Scholar 

  • Unkles SE (1992) Gene organization in industrial filamentous fungi. In: Kinghorn JR, Turner G (eds) Applied molecular genetics of filamentous fungi. Chapman and Hall, London, pp 28–53

    Google Scholar 

  • Wootton JC (1974) The coenzyme-binding domains of glutamate dehydrogenases. Nature 252:542–546

    Google Scholar 

  • Yanisch-Perron C, Vieira J, Messing J (1985) Improved phage M13 cloning vectors and host strains; nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103–109

    Google Scholar 

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Authors and Affiliations

  1. Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreijenlaan 2, NL-6703 HA, Wageningen, The Netherlands

    P. J. Schaap, Y. Müller & J. Visser

  2. Mushroom Experimental Station, PO Box 6042, NL-5960 AA, Horst, The Netherlands

    J. J. P. Baars, A. S. M. Sonnenberg & L. J. L. D. van Griensven

  3. Department of Microbiology and Evolutionary Biology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525 ED, Nijmegen, The Netherlands

    H. J. M. Op den Camp

Authors
  1. P. J. Schaap
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  2. Y. Müller
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  3. J. Visser
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  4. J. J. P. Baars
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  5. A. S. M. Sonnenberg
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  6. L. J. L. D. van Griensven
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  7. H. J. M. Op den Camp
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Communicated by C. A. M. J. J. van den Hondel

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Schaap, P.J., Müller, Y., Visser, J. et al. Nucleotide sequence and expression of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA) fromAgaricus bisporus . Molec. Gen. Genet. 250, 339–347 (1996). https://doi.org/10.1007/BF02174392

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  • DOI: https://doi.org/10.1007/BF02174392

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