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Endo.SK1: an inducible site-specific endonuclease from yeast mitochondria

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Abstract

Site-specific endonucleases have been found in various eukaryotic organelles such as mitochondria, chloroplasts and nuclei. These endonucleases initiate site-specific or homologous gene conversion in mitochondrial and nuclear DNA. Here, we report a new site-specific endonuclease activity, Endo.SK1, identified in mitochondria of strain SK1, a homothallic diploid strain ofSaccharomyces cerevisiae. Nucleotide sequences around the Endo.SK1-cleavage sites are different from those of known yeast site-specific endonucleases. The Endo.SK1 activity is, at least partly, specified by a gene in the SK1-derived mitochondria. A novel feature of the Endo.SK1 activity is its inducibility: the endonuclease activity was induced by ca. 40-fold by transfer of cells from a glucose medium into an acetate medium, and was then repressed. This transient induction was independent of the ploidy level of the cells, and coincided with induction of fumarase, a mitochondrial enzyme involved in the TCA cycle. Co-induction and co-repression of the mitochondrial site-specific endonuclease activity and a respiration-related enzyme indicate that the endonuclease activity is regulated in response to physiological conditions, and suggest a possible role for the endonuclease in mitochondrial DNA metabolism.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, and Biodesign Research Group, Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, 351-01, Saitama, Japan

    K. Ohta, D. Keszenman-Pereyra & T. Shibata

  2. Institut Curie, Section de Biologie, 26 rue d'Ulm, 75231, Paris Cedex 05, France

    A. Nicolas

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  1. K. Ohta
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  2. D. Keszenman-Pereyra
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  3. T. Shibata
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  4. A. Nicolas
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Communicated by R. Devoret

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Ohta, K., Keszenman-Pereyra, D., Shibata, T. et al. Endo.SK1: an inducible site-specific endonuclease from yeast mitochondria. Molec. Gen. Genet. 250, 395–404 (1996). https://doi.org/10.1007/BF02174027

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  • DOI: https://doi.org/10.1007/BF02174027

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