Biotherapy

, Volume 4, Issue 1, pp 69–73 | Cite as

In vitro augmentation of cytotoxicity by N-CWS in peritoneal polymorphonuclear leukocytes (PMNs) induced by PSK, OK-432 or N-CWS

  • Haruki Kato
  • Yoshiharu Yamamura
  • Mamoru Inoue
  • Mari Tanigawa
  • Shigeru Kimura
  • Shigeru Sugino
  • Motoharu Kondo
Article

Abstract

Peritoneal polymorponuclear leukocytes (PMNs) were collected from the peritoneal cavity of C3H/He mice 6 hrs after intraperitoneal (i.p.) injection of 2.5 mg/head of PSK, 1 KE (100 µg)/head of OK-432 or 200 µg/head ofNocardia rubra cell wall skeleton (N-CWS). Withoutin vitro stimulation, these PMNs did not show cytotoxicity to syngeneic MM46 mammary carcinoma cells in51Cr release assay. Cytotoxicity of these PMNs was augmented by the addition of 25 µg/ml of N-CWS but not of PSK or OK-432 to cultures for the assay at the beginning of the culture. H2O2 production of PSK-induced PMNs was increased by thein vitro addition of 25 µg/ml of N-CWS but not of PSK. These results suggest that PSK as well as OK-432 and N-CWS can induce PMNs capable of responding further to N-CWS as the second stimulant.

Key words

N-CWS (Nocardia rubra cell wall skeleton) OK-432 polymorphonuclear leukocytes (PMNs) PSK 

Abbreviations

KE

Klinische Einheit

N-CWS

Nocardia rubra cell wall skeleton

PMNs

polymorphonuclear leukocytes

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Haruki Kato
    • 1
  • Yoshiharu Yamamura
    • 1
  • Mamoru Inoue
    • 1
  • Mari Tanigawa
    • 1
  • Shigeru Kimura
    • 1
  • Shigeru Sugino
    • 1
  • Motoharu Kondo
    • 1
  1. 1.First Department of Internal MedicineKyoto Prefectural University of MedicineKyotoJapan

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