Summary
A method is described for separating the white elements of cow's blood. Addition of water causes a complete haemolysis. About 30 sec later, isotonic conditions are restored by admixing appropriate concentrations of physiological solutions. Differential centrifugation follows for separating the leucocytes.
Literatur
M. Behrens undH. R. Marti, Biochim. biophys. Acta65, 551 (1962).
M. Behrens undM. Taubert, Hoppe-Seylers Z.290, 228 (1952).
P. Szillárd, Pflügers Arch. ges. Physiol.211, 597 (1926).
R. K. Archer, Brit. J. Haemat.6, 229 (1960).
H. Köhler undD. Springer, Zbl. Bakt., I. Abt.180, 264 (1960).
A. Dittmer, F. H. Dost undH. Rind, Klin. Wschr.38, 450 (1960).
E. Klein, S. Eridani, J. Djerassi undR. Resnick, Amer. J. clin. Path.29, 550 (1958).
E. E. Polli, Exper.7, 138 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Behrens, M., Esch, F. Gewinnung von Leukocyten aus Rinderblut unter Verwendung von Wasser als Hämolytikum. Experientia 19, 406–407 (1963). https://doi.org/10.1007/BF02171514
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02171514