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Discelektrophoretische Differenzierung saurer Phosphatasen verschiedener Herkunft

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Summary

Human seminal fluid contents a very high activity of acid phosphatase. Therefore this enzyme attaches medico-legal importance to the evidence of seminal traces. But this enzyme is present in many other biological fluids—even in a very high activity almost like in human seminal fluid. Comparisms of the qualitative reaction on acid phosphatase gave positive results as well with older human seminal spots as with those of fresh fluids of plant or animal origin.

Discelectrophoretical examinations in polyacrylamide gels evidence the heterogeneity of human acid phosphatase and that of plant or animal origin. The best results of separation were obtained by means of the following conditions:

Spacer gel

2.5% Acrylamid

Separating gel

7.5% Acrylamid

Voltage

180 V, 2.5 mA each tubuli

Time of separating

110 min

Cooling the anode puffer with tap water.

Under these conditions in the human seminal fluid could be detected 2 zones of enzyme activity. After a storing periode of 4 weeks in seminal traces on textile spots appears a 3rd zone between the two bands.

Other investigated biological fluids (plants and snails) showed 3–8 zones of enzyme activity.

This bands became unsharp and mixed up after one week of storage.

These investigations implay conditional informations in medico-legal experience of seminal traces.

Zusammenfassung

1. Im menschlichen Ejaculat konnten discelektrophoretisch unabhängig vom Spurenalter konstant 2 Isoenzyme der sauren Phosphatase nachgewiesen werden.

2. Spuren verschiedener Pflanzenpreßsäfte (Studentenblume, Blumenkohl und Schneckenschleime — Weinbergschnecke, Schnirkelschnecke —) zeigten 3–8 Isoenzymbanden. Diese Isoenzyme waren in mehr als 7 Tage alten Spuren nicht mehr scharf zu trennen.

3. Die discelektrophoretische Untersuchung der Isoenzyme der sauren Phosphatase bringt für den gerichtsmedizinischen Spermaspurennachweis weitere wertvolle Informationen.

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  1. Institut für Rechtsmedizin der, Johannes Gutenberg-Universität Mainz (BRD), Mainz

    Peter Höhn, Gotfried Walther & Horst Leithoff

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  1. Peter Höhn
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Höhn, P., Walther, G. & Leithoff, H. Discelektrophoretische Differenzierung saurer Phosphatasen verschiedener Herkunft. Z Rechtsmed 68, 129–137 (1971). https://doi.org/10.1007/BF02169070

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