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Ribonucleaseaktivität isolierter Nebennierenmarkgranula

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Summary

Homogenates from suprarenal medulla of cattle were fractionated by density gradient centrifugation. The catecholamine and RNA content of all fractions obtained, as well as their RN-ase activity, were determined and expressed as % per mg protein. The chromaffin granules contain 49.8% of the catecholamines, 15.7% of the RNA, and 18.7% of the RN-ase activity of all fractions. The demonstration of RN-ase activity in the chromaffin granules is in favour of the hypothesis that this enzyme could catalyse the spontaneous release of RNA, catecholamines, and ATP at 37°C.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Frankfurt am Main, Deutschland

    A. Philippu & H. J. Schümann

Authors
  1. A. Philippu
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  2. H. J. Schümann
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Ausgeführt mit Unterstützung der Deutschen Forschungsgemeinschaft.

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Philippu, A., Schümann, H.J. Ribonucleaseaktivität isolierter Nebennierenmarkgranula. Experientia 20, 547–548 (1964). https://doi.org/10.1007/BF02150281

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