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Solid-phase enzyme immunoassay for detection ofChlamydia trachomatis

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Abstract

The prototype of a solid-phase enzyme immunoassay for the detection ofChlamydia trachomatis antigen was tested in 403 men and 135 women attending a venereal disease clinic. Culture on HeLa 229 cells was used as reference method. In men the overall sensitivity and specificity of the enzyme immunoassay was 70 % and 98.5 % respectively. In the 158 men with non-gonococcal urethritis the sensitivity and specificity was 73 % and 96.5 % respectively, in the 31 men with post-gonococcal urethritis 82 % and 100 %, in the 48 men with gonococcal urethritis 67 % and 100 %, in the 125 men with discharge but normal urinary sediment 54 % and 99.1 % and in the 41 men with no signs or symptoms 50 % and 100 %. In women the sensitivity and specificity of the assay was 70 % and 92 % respectively. On account of the low degree of sensitivity in both men and women and the low degree of specificity in women the prototype of the enzyme immunoassay is not yet considered suitable for clinical use. An improved model is being developed.

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Authors and Affiliations

  1. Department of Dermato-Venereology, University Hospital Rotterdam-Dijkzigt, Dr. Molewaterplein 40, 3015, GD Rotterdam, The Netherlands

    J. van Ulsen, K. H. Tjiam, T. van Joost & E. Stolz

  2. Department of Bacteriology and Clinical Microbiology, University Hospital Rotterdam-Dijkzigt, Dr. Molewaterplein 40, 3015, GD Rotterdam, The Netherlands

    A. van Zuuren-van der Horst, Y. van der Heijst & M. F. Michel

  3. National Institute of Public Health and Environmental Hygiene, Postbox 1, Bilthoven, The Netherlands

    R. V. W. van Eijk

Authors
  1. J. van Ulsen
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  2. A. van Zuuren-van der Horst
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  3. K. H. Tjiam
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  4. Y. van der Heijst
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  5. M. F. Michel
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  6. R. V. W. van Eijk
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  7. T. van Joost
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  8. E. Stolz
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van Ulsen, J., van Zuuren-van der Horst, A., Tjiam, K.H. et al. Solid-phase enzyme immunoassay for detection ofChlamydia trachomatis . Eur. J, Clin. Microbiol. 4, 397–399 (1985). https://doi.org/10.1007/BF02148691

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  • DOI: https://doi.org/10.1007/BF02148691

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