Skip to main content
SpringerLink
Log in

Voltage dependence of calcium current deactivation in the somatic membrane of mouse sensory neurons

  • Published:
Neurophysiology Aims and scope

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Voltage dependence of the deactivation kinetics of calcium inward currents was investigated in the somatic membrane of murine spinal ganglia neurons. It was found that deactivation of high threshold calcium current has a slower component (τ=0.80–0.85 msec at a repolarizing potential of −80 mV) as well as the principal transient exponential component (≤130 μsec at the same potential repolarizing level). A dissimilar relationship exists between amplitudes of the transient and slower exponential components, describing deactivation of high threshold calcium current and degree of activation of the depolarizing shift in membrane potential; the former dependence is expressed by a sigmoid and the latter by a V-shaped curve. The slower component of deactivation of high threshold current was inhibited substantially by perfusing the cell with a Tris-PO4-containing solution. Low-threshold calcium tail current undergoes slower deactivation (τ=1.1–1.2 msec) at a repolarizing potential of −160 mV. A relationship between the time constant of low threshold current deactivation and the type of penetrating cation used was observed. A kinetic model of calcium current deactivation is suggested, taking account of the three different types of calcium channels, (one low and two high threshold) present in the somatic membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. N. S. Veselovskii, P. G. Kostyuk, S. A. Fedulova, and R. E. Shirokov “Deactivation of calcium currents at the soma of spinal ganglion neurons during suppression of depolarizing shift in membrane potential,” Neirofiziologiya,17, No. 5, 682–691 (1985).

    Google Scholar 

  2. P. G. Kostyuk, Ya. M. Shuva, and A. N. Savchenko, “Three types of single calcium channels in the mouse embryo sensory neuronal membrane,” Biomembranes,4, No. 4, 366–373 (1987).

    Google Scholar 

  3. Ya. M. Shuva and A. N. Savchenko, “Single calcium channels in spinal ganglia neurons from newborn rats,” Neirofiziologiya,17, No. 5, 674–682 (1985).

    Google Scholar 

  4. C. M. Armstrong and D. R. Matteson, “Properties of two types of calcium channels in clonal pituitary cells,” J. Gen. Physiol.87, No. 1, 161–182 (1986).

    Article  PubMed  Google Scholar 

  5. A. M. Brown, H. D. Lux, and P. L. Wilson, “A description of activation and conduction in calcium channels based on tail and turn-on current measurements,” J. Physiol.,344, 549–583 (1983).

    PubMed  Google Scholar 

  6. D. P. Corey, J. Dubinsky, and E. A. Schwartz, “The calcium current of rod-photoreceptor inner segments recorded with a whole-cell patch clamp,” Neurosci. Abstr.,8, No. 6, 944 (1982).

    Google Scholar 

  7. G. Cota, “Calcium channel currents in pars intermedia cells of the rat pituitary gland,” J. Gen. Physiol.,88, 83–105 (1986).

    Article  PubMed  Google Scholar 

  8. S. A. Fedulova, P. G. Kostyuk, and N. S. Kostyuk, and N. S. Veselovsky, “Two types of calcium channels in the somatic membrane of new-born rat dorsal root ganglion neurones,” J. Physiol.,359, 431–446 (1985).

    PubMed  Google Scholar 

  9. E. M. Fenwick, A. Marty, and E. Neher, “Sodium and calcium channels in bovine chromaffin cells,” J. Physiol.,331, 599–635 (1982).

    PubMed  Google Scholar 

  10. O. A. Krishtal, V. I. Pidoplichko, and Yu. A. Shakhovalov, “Conductance of the calcium channel in the membrane of snail neurones,” J. Physiol.,210, 423–434 (1981).

    Google Scholar 

  11. H. D. Lux and A. M. Brown, “Patch and whole cell calcium currents recorded simultaneously in snail neurons,” J. Gen. Physiol.,83, No. 5, 727–750 (1984).

    Article  PubMed  Google Scholar 

  12. M. C. Nowycky, A. P. Fox, and R. W. Tsien, “Three types of neuronal calcium channel with different calcium agonist sensitivity,” Nature,316, No. 5902, 440–443 (1985).

    Article  PubMed  Google Scholar 

  13. H. Reuter, C. F. Stevens, R. W. Tsien, and G. Yellen, “Properties of single calcium channels in cardiac cell culture,” Nature,297, No. 5866, 501–504 (1982).

    Article  PubMed  Google Scholar 

Download references

Authors
  1. R. E. Shirokov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 20, No. 2, pp. 185–193, March–April, 1988.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shirokov, R.E. Voltage dependence of calcium current deactivation in the somatic membrane of mouse sensory neurons. Neurophysiology 20, 137–143 (1988). https://doi.org/10.1007/BF02141329

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02141329

Keywords

Search

Navigation