Abstract
One-day-old chicks were infected by aerosolized conidia ofAspergillus fumigatus orA. flavus. Plaques containing viable organisms were observed on the third day of infection. Although plaques persisted for several weeks, cultures could not be recovered from them after about ten days. Flourescent antibody methods revealed an immune response to both agents within three days of challenge. Precipitin titers againstAspergillus active protein (AAP) did not appear until the tenth day, and was not detected after seven weeks. Aspergillus polysaccharide (APS) failed to react antigenically in infected birds. AAP was separated by Sephadex chromatography into nine distinct fractions. One fraction from each species was associated with an immediate and one with a delayed type of skin response in wattles. A low molecular weight fraction which lacked skin reactivity was an effective antigen in precipitin tests. Except for a weak skin reaction toA. fumigatus APS in rabbits, skin tests with extracts and APP fractions correlated with wattle reactions in chicks.
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References
Ajello, L., George, L. K., Kaplan, W. &Kaufman, L. (1968) Laboratory manual for medical mycology. Ed. 2. U.S. Department of Health, Education and Welfare, National Center for Disease Control, Atlanta, Georgia.
Askura, S., Nakagawa, S., Masui, M. &Yasuda, J. (1962) Immunological studies of aspergillosis in birds.Mycopathol. 18:249–256.
Azuma, I., Kimura, H., Hirao, F., Tsubura, E. &Yamamura, Y. (1967) Skin testing and precipitation antigens fromAspergillus fumigatus for diagnosis of aspergillosis.Am. Rev. Resp. Dis. 95:305–306.
Azuma, I., Kimura, H., Hirao, F., Tsubura, E. &Yamamura, Y. (1968) Chemical and immunological properties of protein and polysaccharide fractions extracted fromAspergillus fumigatus.Japan. J. Med. Mycol. 8:210–220.
Azuma, I., Kimura, H., Hirao, F., Tsubura, E. &Yamamura, Y. (1969) Biochemical and immunological studies on Aspergillus. II. Immunological properties of protein and polysaccnaride fractions obtained fromAspergillus fumigatus.Mycopathol. Mycol. Appl. 37:289–303.
Biquet, J., Tran Van Ky, P., Capron, A. &Fruit, J. (1962) Analyse immunochimique des fractions antigeniques solubled'Aspergillus fumigatus. Orde d'apparition des anticorps experiment auz du lapin; comparison de ces derniers avec des anticorps naturels humains.Compt. Rend. Acadm Sci. Paris 254:3768–3770.
Determan, H. (1968) Gel chromatography. Springer-Verlag, Inc. New York.
Fukui, M. &Yasuda, J. (1961) Serological studies onAspergillus fumigatus.Mycopathol. Mycol. Appl. 14:39–54.
Miyazaki, T. (1961) Studies on fungal polysaccharides. I. On the isolation and chemical properties of capsular polysaccharides fromCryptococcus neoformans.Chem. Pharm. Bull. 9:715–718.
Okada, Y., Morisawa, S., Syojima, K., Kitagawa, U., Nakashima, S. &Yamamura, Y. (1963) Improved method for the isolation and properties of tuberculin active peptides.J. Biochem. 54:484–490.
O'Meara, D. C. &Chute, H. L. (1959) Aspergillosis experimentally produced in hatching chicks.Avian Dis. 3:404–406.
Sidransky, H. &Verney, E. (1962) Experimental aspergillosis.Lab. Invest. 11: 1172–1183.
Stanley, N. F. (1949) Biological properties of polysaccharide and lipid fractions from a pathogenic strain ofA. fumigatus.Aust. J. Exptl. Biol. Med. Sci. 28:99–108.
Wright, J. L., Anderson, G. W. &McConachie, J. D. (1961) Transmission of aspergillosis during incubation.Poult. Sci. 40:727–731.
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Taylor, J.J., Burroughs, E.J. Experimental avian aspergilosis. Mycopathologia et Mycologia Applicata 51, 131–141 (1973). https://doi.org/10.1007/BF02141104
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DOI: https://doi.org/10.1007/BF02141104