Skip to main content
Log in

The fate of labelled15N urea and ammonium nitrate applied to a winter wheat crop

I. Nitrogen transformations in the soil

  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

Labelled urea or ammonium nitrate was applied to winter wheat growing on a loamy soil in Northern France. Two applications of fertilizer were given: 50 kg N ha−1 at tillering (early March) and 110 kg N ha−1 at the beginning of stem elongation (mid-April). The kinetics of urea hydrolysis, nitrification of ammonium and the disappearance of inorganic nitrogen were followed at frequent intervals. Inorganic nitrogen soon disappeared, mainly immobilized by soil microflora and absorbed by the crop. Net immobilization of fertilizer N occured at a very similar rate for urea and ammonium nitrate. Maximum immobilization (16 kg N ha1) was found at harvest for the first dressing and at anthesis for the second dressing (23 kg N ha1). During the nitrification period, the labelled ammonium pool was immobilized two to three times faster than the labelled nitrate pool. No significant net15N remineralization was found during the growth cycle.

The actual denitrification and volatilization losses were probably more important than indicated from calculations made by extrapolation of fluxes measured over short intervals. However microbial immobilization was the most important of the processes which compete with plant uptake for nitrogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aulakh M S and Rennie D A 1984 Transformations of fallapplied15N labelled fertilizers. Soil Sci. Soc. Am. J. 48, 1184–1189.

    Google Scholar 

  • Barraclough P B and Leigh R A 1984 The growth and activity of winter wheat roots in the field: The effects of sowing date and soil type on root-growth of high-yielding crops. J. Agric. Sci. Camb. 103, 59–74.

    Google Scholar 

  • Bremner J M 1965a Total nitrogen.In Methods of soil Analysis. Eds. C A Black et al. pp 1149–1178, American Society of Agronomy. Madison, Wisconsin, USA.

    Google Scholar 

  • Chabalier P F, Guiraud G, Pichot J and Remy J C 1975 Evolution de l'azote des engrais dans les sols cultivés: utilisation de l'azote 15″ C.R. fin d'études IRATCU, INRA, CEA, 147 p.

  • Christensen S and Tiedje J M 1988 Denitrification in the field, analysis of spatial and temporal variability. Proceedings of the CEC Seminar ‘Nitrogen Efficiency in Agricultural Soils’, Edinburgh 16–18 Sept. 1987. (In press.)

  • Fresnau C 1982 Contribution à l'étude du devenir de l'urée en sol de limon et en sol calcaire. Thèse d'Université Lyon I, 131 p.

  • Germon J C, Couton Y, Catroux G and Guiraud 1983 Etude des paramètres controlant la dénitrification dans les sols cultivés. Convention de recherche 81–283. Ministère de l'environnement Paris, 23 p.

  • Germon J C, Couton Y, Jacques D, Guiraud G and Catroux G 1985 Etude de la denitrification dans les sols cultivés; effects des facteurs pédologiques, climatiques et culturaux. C.R. congrès ‘Les nitrates dans les eaux’, Paris, 101–108.

  • Henriksen A and Selmer-Olsen A 1970 Automatic methos for determining nitrate and nitrite in water and soils extracts. Analyst 95, 514–518.

    Google Scholar 

  • Kee Kwong N G, Deville J, Cavalot P C and Riviere V 1986 Biological immobilization of fertilizer nitrogen in humid tropical soils of Mauritius. Soil Sci. 141, 3, 195–199.

    Google Scholar 

  • Machet J M, Pierre D, Recous S and Remy J C 1987 Signification du coefficient réel d'utilisation et conséquences pour la fertilisation azotée des cultures. C. R. Acad. Agric. Fr. 73, 3, 39–57.

    Google Scholar 

  • Mahli S S and Nyborg M 1983 Field study of the fate of fall-applied15N-labelled fertilizers in three Alberta soils. Agron J. 75, 71–74.

    Google Scholar 

  • Marshall R B and Whiteway J N 1985 Automation of an interface between a nitrogen analyser and a Isotope Ratio Mass Spectrometer. Analyst 110, 867–871.

    Google Scholar 

  • Mulvaney R L and Bremner J M 1979 A modified diacetyl monoxime method for colorimetric determination of urea in soil extracts. Commun. Soil Sci. Plant Anal. 10, 8, 1163–1170.

    Google Scholar 

  • Nannipieri P, Ciardi C and Palazzi T 1985 Plant uptake, microbial immobilization and residual soil fertilizer of urea nitrogen in a grass-legume association. Soil Sci. Soc. Am. J. 49, 452–457.

    Google Scholar 

  • Nelson D W 1982 Gaseous losses of nitrogen other than through denitrification.In Nitrogen in Agricultural soils. Ed. F T Stevenson. pp 327–358. Agronomy 22. ASA publishers.

  • Neeteson J J, Greenwood D J and Habets E J 1986 Dependence of soil mineral N on N fertilizer application. Plant and Soil 91, 417–420.

    Google Scholar 

  • Olson R V and Swallow C W 1984 Fate of labelled nitrogen fertilizer applied to winter wheat for five years. Soil Sci. Soc. Am. J. 48, 583–586.

    Google Scholar 

  • Okereke G V and Meints V M 1985 Immediate immobilization of labelled ammonium sulfate and urea nitrogen in soils. Soil Sci. 140, 2, 105–108.

    Google Scholar 

  • O'Toole P, Morgan M A and McAleese D M 1982 Effects of soil properties temperature and urea concentration on patterns and rates of urea hydrolysis in some Irish soils. Ir. J. agric. Res. 21, 185–197.

    Google Scholar 

  • Recous S 1988 Dynamique de l'Azote en Sol Cultivé: Organisation Microbienne des Formes Ammoniacale et Nitrique, Conséquences sur l'Utilisation de l'Azote des Engrais par une Culture de Blé. Thèse de doctorat, no. 49/88, U.C.B. Lyon (France), 189 p.

    Google Scholar 

  • Recous S, Machet J M and Mary B 1988 The fate of15N urea and ammonium nitrate applied to a winter wheat crop. II. Plant uptake and N efficiency. Plant and Soil 112, 215–224.

    Google Scholar 

  • Remy J C and Viaux Ph 1982 The use of nitrogen fertilizers in intensive wheat growing in France. Proc. Fertil. Soc. London, 211, 67–92.

    Google Scholar 

  • Ryden J C and Rolston D E 1983 The measurement of denitrification.In Gaseous Loss of Nitrogen From Plant-soil Systems, Developments in Plant and Soil Sciences, vol. 9, Eds. J R Freney and J R Simpson. pp 91–132. M Nijhoff and W Junk Publishers.

  • Rice C W and Smith M S 1984 Short-term immobilization of fertilizer nitrogen at the surface of no-till and ploughed soils. Soil Sci. Soc. Am. J. 48, 295–297.

    Google Scholar 

  • Stewart B A and Porter L K 1963 Inability of the Kjeldahl method to fully measure indigenous fixed ammonium in soils. Soil Sci. 85, 42–46.

    Google Scholar 

  • Verdouw H 1977 Ammonia determination based on indophenol formation with sodiumsalicylate. Water Research, 12, 399–402.

    Google Scholar 

  • Wickramasinghe K N, Rodgers G A and Jenkinson D S 1985 Transformations of nitrogen fertilizers in soil. Soil Biol. Biochem. 17, 5, 625–630.

    Google Scholar 

  • Vlek P L G and Carter M F 1983 The effect of soil environment and fertilizer modifications on the rate of urea hydrolysis. Soil Sci. 136, 1, 56–63.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Recous, S., Fresneau, C., Faurie, G. et al. The fate of labelled15N urea and ammonium nitrate applied to a winter wheat crop. Plant Soil 112, 205–214 (1988). https://doi.org/10.1007/BF02139997

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02139997

Key words

Navigation