Abstract
Development of experimental cataract can be objectively monitored in rats by application of Scheimpflug photography with the SL 45 Topcon camera and subsequent densitometric image analysis. The method has been used to study naphthalene cataract in Brown-Norway rats (BN-CPB), as well as diabetic cataract induced by streptozotocin injection in Sprague-Dawley rats. The values obtained by linear microdensitometric image analysis allowed precise characterization of the opacification with respect to size, topography, and time progress so that statistical evaluation of the efficacy of certain drugs in prevention or delay of experimental cataracts was possible.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrecht M, Barany C (1979) Early lens changes in macaca fascicularis under topical treatment with echothiophate or carbachol studied by slit image photography. Invest Ophthalmol Vis Sci 18: 179–187
Bölingen B (1983) Die Anwendung der densitometrischen Bildanalyse von Scheimpflug-Photographien bei tierexperimentellen Kataraktformen. (Naphthalinkatarakt bei Brown-Norway-Ratten). Diss, Bonn
Chandler, M.L., Boltralik, J., York, B., and DeSantis, L.: Prevention of cataracts in diabetic rats by aldose reductase inhibitors. Invest. Ophthalmol. Vis. Sci.22, 156 (1982)
Dobbs R, Smith J, Knowles W, Hockwin O (1984) Long-term follow-up of lens changes in diabetic patients. Invest Ophthalmol Vis Sci ARVO (Suppl) 25; No 3, 270 (Abstr)
Dragomirescu V, Hockwin O, Koch H-R, Sasaki K (1978) Development of a new equipment for rotating slit image photography according to Scheimpflug's principle. Interdiscip Top Gerontol 13:118–130
Dragomirescu V, Hockwin O, Koch H-R (1980) Photo-cell device for slit beam adjustment to the optical axis of the eye in Scheimpflug-photography. Ophthalmic Res 12:78–86
Hockwin O, Dragomirescu V (1981) Die Scheimpflug-Photographie des vorderen Augenabschnittes. Eine Methode zur Messung der Linsentransparenz im Rahmen einer Verlaufsbeobachtung. Z Prakt Augenheilkd 2:129–136
Hockwin O, Dragomirescu V (1982) Verlaufsbeobachtungen von Linsentrübungen mit der Scheimpflug-Photographie und densitometrischer Bildanalyse. In: Hockwin O (ed) Altern der Linse, Symposium Strasbourg 1982. Mayr, Miesbach, 128–138
Hockwin O, Dragomirescu V, Laser H (1983a) Measurements of lens transparency or its disturbances by densitometric image analysis of Scheimpflug photographs. Graefe's Arch Clin Exp Ophthalmol 219:255–262
Hockwin O, Weigelin E, Laser H, Dragomirescu V (1983b) Biometry of the anterior eye segment by Scheimpflug photography. Ophthalmic Res 15:102–108
Hockwin O, Dragomirescu V, Laser H (1983c) Age related changes in normal and cataractous human lens density obtained by microdensitometric image analysis of Scheimpflug-photographs. Lens Research 1 (1&2):207–220
Hockwin O, Dragomirescu V, Laser H, Ohrloff C, Kozamanoglu K, Kremer F (1984a) Evaluation of ocular safety of verapamil. Scheimpflug photography with densitometric image analysis of lens transparency in patients with hypertrophic cardiomyopathy (HOCM) subjected to long-term therapy with high doses of verapamil. Ophthalmic Res 16
Hockwin O, Dragomirescu V, Shibata T, Laser H (1984b) Monitoring experimental cataracts of rats by Scheimpflug photography during long-term follow-up examinations. (Exhib.) 24. Meeting AER, Wien, 1983, Abstr Ophthalmic Res 16:228
Hockwin O, Wegener A, Micic L, Kaiser J, Shibata T, Schwarz B, Müller P, Ahrend M, Bours J, York B (1984c) Streptozotocin diabetes and effect of aldose reductase inhibitor AL-1567. 24. Meeting AER, Wien 1983, Abstr. Ophthalmic Res 16:208
Lerman S, Hockwin O (1981) UV-visible slit lamp densitography of the human eye. Exp Eye Res 33:587–596
Lerman S, Hockwin O, Dragomirescu V (1981) In vivo lens fluorescence photography. Ophthalmic Res 13:224–228
Lerman S, Dragomirescu V, Hockwin O (1982) In vivo monitoring of direct and photosensitized UV radiation damage to the lens. XXIV International Congress of Ophthalmology, November 1982, San Francisco. ACTA, Vol 1:354–358, Ophthalmology 89:Suppl. 101 Abstr
Mayer H, Barth K, Horrer E (1984) Therapeutische Probleme beim Grauen Altersstar (in press)
NIH-Publication No. 83-2473 (1983) “Vision Research, a National Plan 1983–1987”. Report of the Cataract Panel. Vol 2/pt 3:52, 91
Olbert D, Blassmann K (1983) Zur klinischen Anwendung der Scheimpflug-Fotografie am vorderen Augenabschnitt. Klin Monatsbl Augenheilk 182:96–98
Weigelin E, Hockwin O (1982) Bericht über eine zufallsverteilte, kontrollierte klinische Studie mit Phakan/Phakolen. In: Hockwin O (ed) Altern der Linse, Symposium Strasbourg 1982. Mayr, Miesbach, 183–199
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hockwin, O., Dragomirescu, V., Shibata, T. et al. Long-term follow-up examination of experimental cataracts in rats by Scheimpflug photography and densitometry. Graefe's Arch Clin Exp Ophthalmol 222, 20–25 (1984). https://doi.org/10.1007/BF02133772
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02133772