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Prevention of vascular graft infection by rifampin bonding to a gelatin-sealed dacron graft

  • Original Articles
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Annals of Vascular Surgery

Abstract

This study examines the efficacy of rifampin bonding to a gelatin-sealed knitted Dacron graft to prevent perioperative bacteremic vascular graft infection. Antibiotic bonding was obtained by soaking grafts for 15 minutes in a 1 mg/ml saline solution of rifampin at 37°C. Nineteen dogs had thoracoabdominal aortic bypass: seven (group I) received a rifampin treated graft; six (group II) received an untreated gelatin-coated graft; and six (group III) received an uncoated Dacron graft. Two days later bacteremic challenge was produced by rapid intravenous injection of 5×10 5 colony forming units of methicillin resistantStaphylococcus aureus.Grafts were harvested five days after this challenge and cut into 10 fragments, each submitted to bacterial counts. Results were expressed as CFU/cm 2 of graft material. In group I, no graft was infected, whereas all grafts in groups II and III were infected (p<0.05). Median bacterial counts from the infected fragments (median±SD) were similar in groups II (2.5×105 CFU/cm2) and III (4×104 CFU/cm2). Blood cultures at time of sacrifice were negative in all dogs in group I and positive in five of six dogs in groups II and III. Cultures of liver, spleen, kidney, and lung specimens were always negative in group I and positive in 22 of 24 specimens in group II and 23 of 24 specimens in group III. Soaking a gelatin-sealed Dacron graft in rifampin solution evidently prevents early bacteremic graft infection and secondary foci of infection in this model.

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Authors and Affiliations

  1. Groupe d'Etude des Biomatériaux, Departments of Surgery and Anesthesiology, Ambroise Paré Hospital and René Descartes University, France

    Olivier Goëau-Brissonnière MD, PhD, Catherine Leport MD, PhD, François Bacourt MD, Claude Lebrault MD, Raymonde Comte & Jean -Claude Pechère MD

  2. Department of Infectious and Tropical Diseases, Claude Bernard Hospital, INSERM U 13, Paris, France

    Olivier Goëau-Brissonnière MD, PhD, Catherine Leport MD, PhD, François Bacourt MD, Claude Lebrault MD, Raymonde Comte & Jean -Claude Pechère MD

  3. Department of Microbiology, Faculty of Medicine, Geneva, Switzerland

    Olivier Goëau-Brissonnière MD, PhD, Catherine Leport MD, PhD, François Bacourt MD, Claude Lebrault MD, Raymonde Comte & Jean -Claude Pechère MD

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  1. Olivier Goëau-Brissonnière MD, PhD
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  2. Catherine Leport MD, PhD
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  3. François Bacourt MD
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  4. Claude Lebrault MD
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  5. Raymonde Comte
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  6. Jean -Claude Pechère MD
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Goëau-Brissonnière, O., Leport, C., Bacourt, F. et al. Prevention of vascular graft infection by rifampin bonding to a gelatin-sealed dacron graft. Annals of Vascular Surgery 5, 408–412 (1991). https://doi.org/10.1007/BF02133043

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