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Culturing and extraction ofCoprococcus comes, absorption of serumagglutinins by soluble fractions and relation between agglutinins and antibodies in sera of patients with Crohn's disease

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Abstract

Agglutinating antibodies toCoprococcus comes and three other obligately anaerobic coccoid rods from the intestinal flora are used in the diagnosis of Crohn's disease. Further studies on the pathogenetic role as well as the development of more sensitive and specific methods for detecting antibodies require extraction of the antigen fractions. Culturing methods to obtainC. comes with optimal antigen presentation and isolation of soluble antigen fractions were therefore developed. Hot water extraction of whole cells and subsequent removal of proteins with trichloroacetic acid provided a fraction that (1) absorbed serum agglutinins, (2) was useful for an enzyme-linked immunosorbent assay and (3) induced agglutinating antibodies in rats.

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Authors and Affiliations

  1. Department of Medical Microbiology, Erasmus University Rotterdam, P.O. Box 1738, 3000, DR Rotterdam, The Netherlands

    M. P. Hazenberg, A. M. Pennock-Schröder & J. P. van de Merwe

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  1. M. P. Hazenberg
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  2. A. M. Pennock-Schröder
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  3. J. P. van de Merwe
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Hazenberg, M.P., Pennock-Schröder, A.M. & van de Merwe, J.P. Culturing and extraction ofCoprococcus comes, absorption of serumagglutinins by soluble fractions and relation between agglutinins and antibodies in sera of patients with Crohn's disease. Med Microbiol Immunol 175, 299–306 (1986). https://doi.org/10.1007/BF02126051

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  • DOI: https://doi.org/10.1007/BF02126051

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