Summary
UV-irradiation of SV 40 was shown to reduce considerably the infectivity, the ability to induce T-antigen, and the induction of DNA synthesis. Oncogenicity, however, was reduced only slightly “in vitro” and “in vivo”. The ability of SV 40 to replicate, to form T-antigen, and to induce DNA synthesis do not seem to be necessary conditions for oncogenic potency “in vitro” or “in vivo”. Several interpretations, as well as the possible implication for vaccine-production, are discussed.
Zusammenfassung
Das SV40-Virus zeigte nach UV-Bestrahlung eine erhebliche Reduktion des Infektionstiter, sowie eine starke Abnahme der Fähigkeit zur T-Antigenbildung und Induktion der DNS-Synthese. Die onkogene Wirksamkeit war dagegen nach UV-Bestrahlung des SV40-Virus nur geringfügig „in vitro“ und „in vivo“ vermindert. Die Fähigkeiten des SV40-Virus zur Replikation, T-Antigenbildung und Induktion der DNS-Synthese scheinen keine unbedingten Voraussetzungen für die onkogene Wirksamkeit „in vitro“ und „in vivo“ darzustellen. Verschiedene Deutungsmöglichkeiten, die dieses Phänomen erklären könnten, werden diskutiert. Auf die mögliche Bedeutung dieser Ergebnisse für die Impfstoffherstellung wird hingewiesen.
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Die Untersuchungen wurden mit Unterstützung der Deutschen Forschungsgemeinschaft im Rahmen der Unit „Medizinische Virologie” durchgeführt.
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Seemayer, N., Seemayer, G. & Haas, R. Untersuchungen über die onkogene Transformation, Induktion der DNS-Synthese und T-Antigenbildung durch UV-bestrahltes Simian Virus (SV-) 40. Z. med. Mikrobiol. u. Immunol. 155, 123–132 (1969). https://doi.org/10.1007/BF02123856
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DOI: https://doi.org/10.1007/BF02123856