Abstract
Early nuclear antigen (CMNA) induced by human cytomegalovirus (HCMV) was extracted from infected human embryonic fibroblast cells and purified by ds DNA Sephadex chromatography. The purified antigen was added to acid-fixed preparations from human embryonic fibroblasts and these in vitro converted nuclei were exposed to human sera to estimate the antibodies to CMNA by anti-complement immunofluorescence staining. Serial serum samples were obtained from eight patients suffering from acute HCMV infection (mononucleosis), from nine patients with Epstein-Barr virus (EBV) mononucleosis, and from 20 healthy persons who had been shown to possess antibodies to HCMV late antigens. Acute HCMV infection is characterized by the presence of anti-CMNA antibodies at high titer (1∶16–1∶32) together with the elevated level of IgG antibodies to anti-HCMV-late antigens. During convalescence the anti-CMNA titer decreased to a lower level which was maintained for long period.
The anti-CMNA antibodies were also regularly detected in sera of persons possessing antibodies to HCMV late antigens but without any sign of acute HCMV infection. It is concluded that antibodies to CMNA are not transitory and their presence even at high titer (1∶16–1∶32) in a serum sample cannot be taken as a presumptive evidence of acute virus infection.
This method seems to be valuable for measuring antibodies to nuclear DNA-binding antigens induced by other viruses.
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References
Bearden JC Jr (1980) Isolation of nucleolar DNA-binding proteins by simultaneous, competitive DNA-Sephadex affinity chromatography. J Biochem Biophys Methods 2:37–47
Geder L (1976) Evidence for early nuclear antigens in Cytomegalovirus-infected cells. J gen Virol 32:315–319
Gergely L, Czeglédy J, Szalka A, Váczi L, Binder L (1977) Epstein-Barr virus and Cytomegalovirus antibodies in infectious mononucleosis. Acta microbiol Acad Sci hung 24:13–19
Gergely L, Czeglédy J, Váczi L (1980) Early nuclear antigen as DNA-binding protein in Cytomegalovirus infected cells. Intervirology 13:352–356
Kamata T, Tanaka S, Watanabe Y (1978) Human CMV-induced chromatin factors responsible for changes in template activity and structure of infected cell chromatin. Virology 90:197–208
Michelson-Fiske S, Horodniceanu F, Guillon JC (1977) Immediate early antigens in Human Cytomegalovirus infected cells. Nature 270:615–617
Musiani M, Zerbini M, Landini MP, La Placa M (1979) Preliminary evidence that Cytomegalovirus-induced immediate early antigens are DNA-binding proteins. Microbiologia 1:281–287
Ohno S, Luka J, Lindahl T, Klein G (1977a) Identification of a purified complement-fixing antigen as the Epstein-Barr virus-determined nuclear antigen (EBNA) by its binding to metaphase chromsomes. Proc nat Acad Sci (Wash) 74:1605–1609
Ohno S, Luka J, Falk L, Klein G (1977b) Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative herpesvirus papio (HPV)-transformed lymphoid lines by the acid fixed nuclear binding technique. Int. J Cancer 20:941–946
Ohtsuka M, Tanaka S, Saitho H, Hashimoto K (1979) Seroconversion to Human Cytomegalovirus-specific pre-early nuclear antigens in renal transplant recipients. Microbiol. Immunol 23:349–355
Rapp F, Rasmussen LE, Benyesh-Melnick M (1963) The immunofluorescence focus technique in studying the replication of CMV. J Immunol 91:709–719
Reynolds DW (1978) Development of early nuclear antigen in Cytomegalovirus infected cells in the presence of RNA and protein synthesis inhibitors. J gen Virol 40:475–480
Tanaka S, Otsuka M, Ihara S, Maeda F, Watanabe Y (1979) Induction of pre-early nuclear antigen(s) in HEL cells infected with Human Cytomegalovirus. Microbiol Immunol 23:263–271
The TH, Klein G, Langenhuysen MMAC (1974) Antibody reactions to virus-specific early antigens (EA) in patients with Cyotmegalovirus (CMV) infection. Clin exp Immunol 16:1–12
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Gergely, L., Czeglédy, J. & Váczi, L. Complement-fixing antibodies to human cytomegalovirus induced early nuclear antigens in mononucleosis. Med Microbiol Immunol 170, 99–108 (1981). https://doi.org/10.1007/BF02122674
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DOI: https://doi.org/10.1007/BF02122674