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Synthetic and biological polycations in prolongation of skin allograft survival time

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Summary

The degree of immuno-auppressive activity of the lysine-rich F1-fraction and the arginine-rich F3-fraction of histone extracted from calf thymus nuclei was compared with that of total histone and of synthetic polycations using transplantation immunity as experimental model. Under comparable conditions total histone and the F1-fraction were likewise active in prolongation of allograft survival time, whereas the F3-fraction was significantly less active. Poly-l-lysine induced also an inhibition of graft rejection, but its activity was less pronounced compared with the F1-fraction. Poly-l-arginine showed no activity at all.

In addition, the immuno-suppressive effect of total histone extracted from different inbred lines of mice was tested comparatively. Apart from a general activity, it was found in each of four series with different graft-receptor-relationships that an optimal prolongation of skin allograft survival time was uniformly observed, when graft recipients were pretreated with histone from the line of the graft donor or from an unrelated inbred line of the same species. In contrast, in each case pretreatment of graft recipients with histone derived from the line of the recipient was markedly less effective.

Zusammenfassung

Am Modell der Haut-Homotransplantation wurde der immunosuppressive Effekt der lysinreichen F1- und der argininreichen F3-Fraktion von Histon aus Zellkernen von Kalbsthymus mit dem von Gesamthiston und von synthetischen Polycationen verglichen. Unter vergleichbaren Bedingungen bewirkte Gesamthiston und die F1-Fraktion eine ähnliche Verlängerung der Haftung von Haut-Homotransplantaten. Dagegen war die F3-Fraktion signifikant weniger wirksam. Auch Poly-l-Lysin bewirkte eine Hemmung der Transplantat-Abstoßung, doch war dieser Effekt weniger ausgeprägt als der von F1-Histon. Poly-l-Arginin war unwirksam.

Außerdem wurde Gesamthiston, das von verschiedenen Inzuchtlinien von Mäusen gewonnen wurde, im Hinblick auf eine Hemmung der Transplantat-Abstoßung verglichen. Von einer allgemeinen Histon-Wirkung abgesehen, hatte in 4 Versuchsserien mit verschiedenen Spender-Empfänger-Kombinationen jeweils das Histon der Linie des Transplantat-Spenders oder das einer dritten Inzuchtlinie von Mäusen den besten Effekt. Dagegen war in jedem Fall das Histon des Transplantat-Empfängers nennenswert weniger wirksam.

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, Technische Hochschule, 5100, Aachen, GFR (German Federal Republic)

    G. Gillissen

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  1. G. Gillissen
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Additional information

Supported by Grant Gi 27/9 of the „Deutsche Forschungsgemeinschaft“.

The technical assistance of Miss M. Müllenmeister and of Miss Ch. Möller is gratefully acknowledged.

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Gillissen, G. Synthetic and biological polycations in prolongation of skin allograft survival time. Med Microbiol Immunol 157, 17–23 (1971). https://doi.org/10.1007/BF02121287

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