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Electron-microscopic identification of infectious particles of lymphocytic choriomeningitis

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Abstract

LCM virus, strain WE-grown on L cells- and labeled with3H-uridine was centrifuged to equilibrium in a sucrose density gradient and examined in fractions for infectivity, incorporated radioactivity, and electron-microscopic features. The peak of infectivity is congruent with the one of radioactivity (density=1.17 g/ml). LCM virus specificity of the radioactive peak was proved by precipitation of the radioactivity with anti-LCM virus antiserum. The peak fractions showed an abundance of 106±14 nm (1 s) particles. They could be agglutinated with specific anti-LCM virus antiserum but not with antiserum directed against the histocompatibility (H-2) antigens of L cells.

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Authors and Affiliations

  1. Department of Medical Microbiology, Institute of Hygiene, Georg-August-University Göttingen, Germany

    M. Blechschmidt & R. Thomssen

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  1. M. Blechschmidt
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  2. R. Thomssen
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Supported by a grant of the Bundesministerium für Jugend, Familie und Gesundheit, Bonn.

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Blechschmidt, M., Thomssen, R. Electron-microscopic identification of infectious particles of lymphocytic choriomeningitis. Med Microbiol Immunol 162, 193–199 (1976). https://doi.org/10.1007/BF02120997

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