Summary
Blood group A-active substance was extracted from urea-treated human hair with methanol-ethyl ether 1:1, v/v) or chloroform-methanol (1:1, v/v). The serological activity of blood group A substance in the hair was destroyed by A-decomposing enzyme fromClostridium tertium with concomitant development of blood group H activity. It is concluded therefore that the extract from the hair of group A contained blood group A-active glycolipid withN-acetylgalactosamine as the non-reducing sugar.
Zusammenfassung
Das A-blutgruppenaktive Glycolipoid wurde mit Methanol-Äther (1:1, v/v) oder Chloroform-Methanol (1:1, v/v) aus Harnstoff-behandelten Haaren extrahiert. Durch Einwirkung von A-zerstörendem Enzym ausClostridium tertium auf das A-Glycolipoid aus Haaren, kommt nunmehr die H-Eigenschaft nach Inaktivierung von A zum Vorschein. Darum kann essein, daßN-Acetyl-Galactosamin für die A-Aktivität vom Haarglycolipoid benötigt wird.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Yada, S., Okane, M., Sano, Y.: Blood grouping of a single human hair by means of elution technique. Acta Crim. Jap.32, 7–8 (1966)
Sakai, T.: Studies on blood group substances of human hairs (Japanese). Jap. J. Leg. Med.5, 19–41 (1951)
Steinert, P. M., Rogers, G.: Characterization of the proteins of guinea-pig hair and hair-follicle tissue. Biochem. J.135, 759–771 (1973)
Morgan, W. T. J., King, H. K.: Studies in immunochemistry. 7. The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A specificity. Biochem. J.37, 640–651 (1943)
Takizawa, H.: Blood group substances of human erythrocyte. 1. Sugar structures of blood group-active oligosaccharides from stroma by means of ozonolysis (Japanese). Kitakanto Med. J.20, 171–194 (1970)
Iseki, S., Okada, S.: On a specific enzyme which decomposes group A substances. Proc. Jap. Acad.27, 455–458 (1951)
Yamamoto, H., Iseki, S.: Development of the H specificity in A substance by A-decomposing enzyme fromClostridium tertium A. Proc. Jap. Acad.44, 263–268 (1968)
Iseki, S., Furukawa, K., Yamamoto, S.: B substance-decomposing enzyme produced by an anaerobic bacterium. I. Serological action on the B-decomposing enzyme. Proc. Jap. Acad.35, 507–512 (1959)
Iseki, S., Tsunoda, S.: On a bacterial enzyme which specifically decomposes O substance. Proc. Jap. Acad.28, 370–373 (1952)
Iseki, S., Yamamoto, S., Furukawa, K.: A new O (H) substance-decomposing enzyme produced by an aerobic bacterium. II. Chemical action of the O (H)-decomposing enzyme. Proc. Jap. Acad.38, 57–62 (1962)
Iseki, S.: Glycosidases and serological changes in blood group substances. In: Blood and tissue antigesn (Aminoff, D. ed.). pp. 379–394, New York: Academic Press Inc. 1970
Marcus, D. M., Kabat, E. A., Schiffman, G.: Immunochemical studies on blood groups. XXXI. Destruction of blood group A activity by an enzyme fromClostridium tertium which deacetylatesN-acetylgalactosamine in intact blood group substances. Biochemistry3, 437–443 (1964)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kishi, K., Iseki, S. Immunochemical studies on blood group A substance from human hair. Z Rechtsmed 80, 191–195 (1977). https://doi.org/10.1007/BF02114615
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02114615