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Effect of D- and L-1,3-butanediol isomers on glycolytic and citric acid cycle intermediates in the rat brain

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Abstract

DL-1,3-butanediol (DL-BD) is an ethanol dimer which affords cerebral protection in various experimental models of hypoxia and ischemia but its mechanism of action is unknown. DL-BD is a ketogenic alcohol and it has been proposed that its protective effect was accomplished through cerebral utilization of ketone bodies. Since DL-BD is a racemic, its metabolic effects could be due to D, L or both isomers. The effects of equimolar doses of DL-, D- and L-BD (25 mmol/Kg) on cerebral metabolism were studied by measuring the cortical levels of the main glycolytic (glycogen, glucose, glucose 6-phosphate, fructose 1,6-diphosphate, pyruvate and lactate) and citric acid cycle (citrate, alpha-ketoglutarate and L-malate) intermediates. The two BD isomers exerted different effects on cerebral metabolism. Unlike L-BD, D- and DL-BD treatments resulted in a slight (+10%) but significant increase in citrate level whereas L-BD treatment led to significant reduction in pyruvate (-12%) and lactate (-24%) levels. These effects were apparently not linked to hyperketonemia, since DL-BHB treatment, which mimicked hyperketonemia induced by DL-BD, had no effect on cerebral metabolites but might be due to intracerebral metabolism of BD.

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  1. Laboratoire de Pharmacodynamie, Faculté de Pharmacie, 7, Boulevard Jeanne d'Arc, 21033, Dijon Cedex, France

    Serge Gueldry & Jean Bralet

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  1. Serge Gueldry
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  2. Jean Bralet
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Gueldry, S., Bralet, J. Effect of D- and L-1,3-butanediol isomers on glycolytic and citric acid cycle intermediates in the rat brain. Metab Brain Dis 10, 293–301 (1995). https://doi.org/10.1007/BF02109360

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  • DOI: https://doi.org/10.1007/BF02109360

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