Zusammenfassung
Mit Hilfe einer Immunglobulin-Enzym-Brücken-(PAP-) Technik wurde die Lokalisation von zwei neuen ubiquitären Gewebsproteinen (PP8, PP9) und von drei neuen plazentaspezifischen Gewebsproteinen (PP10, PP11, PP12) im Gewebe reifer menschlicher Plazenten untersucht:
PP8 war hauptsächlich im Zytoplasma und in den Zellkernen der Trophoblastzellen (Chorion) und des Synzytiotrophoblasten (Villi) lokalisiert; PP9 im Zytoplasma der Trophoblastzellen, im fibrösen Teil des Stromas (Villi) und im Zytoplasma von Histiozyten (Villi, Amnion und Dezidua), PP10 im Zytoplasma des Epithels (Amnion) sowie im Zytoplasma und in Zellkernen des Synzytiotrophoblasten (Villi), PP12 im Zytoplasma von Histiozyten in Dezidua, Amnion und Chorion sowie im Zytoplasma des Syncytiotrophoblasten (Villi). PP11 wurde ausschließlich im Zytoplasma von Trophoblastzellen (Chorion und Villi) aufgefunden und scheint für den Trophoblast hochspezifisch zu sein, während PP8, PP10 und PP12 zusätzlich im Zytoplasma einiger Histiozyten des Amnions, der Villi, des intervillösen Raumes und der Dezidua nachgewiesen wurden. Die mögliche klinische Bedeutung dieser Plazentaproteine wird diskutiert.
Summary
By using an immunoglobulin enzyme bridge (PAP) technique, the location of two ubiquitous tissue proteins (PP8, PP9) and three placental specific tissue proteins (PP10, PP11, PP12) was investigated in ethanol/acetic acid-fixed paraffin-embedded human term placentae.
PP8 was found mainly in both the cytoplasm and the nucleus of trophoblast cells (chorion) and syncytiotrophoblast (villi). PP9 was found in the cytoplasm of trophoblast cells (chorion), the fibrous part of interstitial connective tissues (villi), and the cytoplasm of histiocytes (villi, amnion, and decidua). PP10 was found in the cytoplasm of epithelium (amnion) and in both the cytoplasm and the nucleus of syncytiotrophoblast (villi). PP12 was found in the cytoplasm of histiocytes in decidua, amnion, and chorion and the cytoplasm of syncytiotrophoblast (villi). In addition, stains for PP8, PP10, and PP12 were taken up by the cytoplasm of histiocytes found in amnion, villi, the intervillous space, and decidua, in contrast to PP11, which was found to be specific to the cytoplasm of trophoblast cells (chorion and villi). Possible clinical applications of these findings are discussed.
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Inaba, N., Renk, T. & Bohn, H. Immunohistochemical location of placental proteins (PP8, 9, 10, 11, 12) in human term placentae. Arch. Gynecol. 230, 109–121 (1980). https://doi.org/10.1007/BF02108266
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DOI: https://doi.org/10.1007/BF02108266
Schlüsselwörter
- Ubiquitäre Gewebsproteine (PP8, PP9)
- Plazentaspezifische Gewebsproteine (PP10, PP11, PP12)
- Reife menschliche Plazenta
- Immunglobulin-Enzym-Brücken-(PAP-)Technik
- Immunhistochemische Lokalisation