Abstract
Regarding some saprophytic, atypical and tuberculous mycobacteria it has been studied the effect of the oleic acid added to the Kirchner's medium. The oleic acid alone, added to the Kirchner's medium, inhibits the tuberculous and some atypical mycobacterial growth.
The addition of oleic acid together with the albumin fraction V from bovine plasma to the medium, scarcely affects the tuberculous mycobacterial growth; on the contrary, it stimulates the atypical mycobacterial production during all the period of growth, and greatly reduces the latency period.
The increase of oxygen consumption due to the oleic acid, is evident on the atypical mycobacteria and particularly on the younger cells, during their first stage of growth, when the olcic acid stimulant effect on the bacterial culture is greater.
Instead, the increase of the oxygen consumption due to the oleic acid is much less evident on the saprophytic and tuberculous mycobacteria and it is particularly notable when the culture growth is at the end. The antimycobacterial effect of the PAS becomes increased with the oleic acid, while it is greatly reduced with the “oleic-methionine acid” system.
These diversified behavior are a further useful data contributing to the mycobacterial differentiation.
Résumé
Les Auteurs ont étudié l'effet de l'acide oléique ajouté au milieu de Kirchner vis-à-vis de quelques souches de mycobactéries tuberculeuses, saprophytes et “atypiques”. L'acide oléique, ajouté seul au milieu de Kirchner exerce une inhibition sur la croissance des mycobactéries et de quelques “atypiques”. L'acide oléique, ajouté au milieu avec la fraction V de l'albumine de boeuf, influence seulément peu la croissance des mycobactéries tuberculeuses, stimule au contraire la multiplication des mycobactéries “atypiques” pendant toute la courbe de croissance et raccourcit nettement la période de latence.
L'acide oléique provoque une évidente augmentation de la consommation d'oxygène sur les mycobactéries “atypiques” et particulièrement sur les cellules jeunes pendant les premières phases de croissance quand l'effet de stimulation de l'acide oléique sur le développement de l'activité bactérienne est plus élevé.
Pour les mycobactéries tuberculeuses et saprophytes l'augmentation de la consummation d'oxygène due à l'acide oléique est au contraire beaucoup moins évidente et cela est observé surtout quand la culture est à la fin de la croissance. L'acide oléique augmente l'activité anti-mycobactérienne du P.A.S. pendant que le système “acide oléique-méthionine” la diminue nettement.
Cettes différences d'allure constituent une donnée ultérieure pour contribuer à la différentiation de mycobactéries.
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Mariani, B., Velluti, G. & Covacev, L. Effets de quelques acides gras nonsaturés sur les mycobactéries. Pneumologie 142, 256–263 (1970). https://doi.org/10.1007/BF02095223
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DOI: https://doi.org/10.1007/BF02095223