Abstract
Protoplasts have been isolated from young vegetative mycelia ofAgaricus bisporus by an enzyme mixture of novozym and chitinase. Protoplasts were released through ruptures in the wall, initially at the apices, but also later from older parts of the hyphae, indicating that they may lack the cell wall. The process of regeneration of these protoplasts has been investigated in liquid medium in which the protoplasts produced short chains of convoluted cells that finally produced a hypha. Electron microscopy has shown that at the start of regeneration two different kinds of fibrils were produced at the external surface of the protoplasts. Later, the thickness of the cell wall increased, and there was a deposit of amorphous material giving rise to a complete new wall.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Carbonell LM, Gil F, Yegres F (1973) Cell wall regeneration of protoplasts ofHistoplasma capsulatum. In: Villanueva JR, García Acha I, Gascón S, Uruburu F (eds) Yeast, mould and plant protoplasts. London, New York: Academic Press, pp 93–103
García Mendoza C, Avellán MA, Sánchez E, Novaes-Ledieu M (1987) Differentiation and wall chemistry ofAgaricus bisporus vegetative and aggregated mycelia. Arch Microbiol 148:68–71
García Mendoza C, Sánchez E, Novaes-Ledieu M (1987) Differences in microfibrils in the walls ofAgaricus bisporus secondary mycelium. FEMS Microbiol Lett 44:161–165
Michalenko GO, Hohl HR, Rast D (1976) Chemistry and architecture of the mycelial wall ofAgaricus bisporus. J Gen Microbiol 92:251–262
Novaes-Ledieu M, Martínez Cobo JA, García Mendoza C (1987) The structure of the mycelial wall ofAgaricus bisporus. Microbiologia SEM 3:13–23
Peberdy JF (1979) Fungal protoplasts: isolation, reversion and fusion. Annu Rev Microbiol 33:21–39
Pérez Cabo A, Reyes E, Novaes-Ledieu M, García Mendoza Mendoza C (1986) Cell wall remnants and spheroplast regeneration inMucor rouxii. Trans Brit Mycol Soc 86:545–549
Song GH, Cho KY (1987) A synthetic medium for the production of submerged cultures ofLentinus edodes. Mycologia 79:866–876
Sonnenberg ASM, Wessels JGH (1987) Heterokaryon formation in the basidiomyceteSchizophyllum commune by electrofusion of protoplasts. Theor Appl Genet 74:654–658
Strunk C (1970) Protoplasts of moulds—the present state of knowledge. In: Necas O. Svoboda A (eds) Yeast protoplasts. Proc Int Symp Brno. Brno University, JE Purkyne, pp 107–119
Villanueva JR, García Acha I (1971) Production and use of fungal protoplasts. In: Booth C (ed) Methods in microbiology, vol 4. New York: Academic Press, pp 665–718
Vries OMH De, Wessels JGH (1972) Release of protoplasts fromSchizophyllum commune by a lytic enzyme preparation fromTrichoderma viride. J Gen Microbiol 73:13–22
Vries OMH De, Wessels JGH (1973) Release of protoplasts fromSchizophyllum commune by combined action of purified α-1,3 glucanase and chitinase derived fromTrichoderma viride. J Gen Microbiol 76:319–330
Vries OMH De, Wessels JGH (1975) Chemical analysis of cell wall regeneration and reversion of protoplasts fromSchizophyllum commune. Arch Microbiol 102:209–218
Wessels JGH, Hoeksema HL, Stemerding D (1976) Reversion of protoplasts from dikaryotic mycelium ofSchizophyllum commune. Protoplasma 89:317–321
Wu L-C (1987) Strategies for conservation of genetic resources. In: Wuest PJ, Royse DJ, Beelman RB (eds) Cultivating edible fungi. Proc Int Symp (IMS 86), Amsterdam, Oxford, New York, Tokyo: Academic Press, pp 183–211
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mendoza, C.G., Cabo, A.P., González, M.L.S. et al. Morphological and ultrastructural studies on protoplast production and reversion of the higher basidiomyceteAgaricus bisporus . Current Microbiology 22, 191–194 (1991). https://doi.org/10.1007/BF02092133
Issue Date:
DOI: https://doi.org/10.1007/BF02092133