Skip to main content
SpringerLink
Log in

Enhanced protein synthesis in the renal arteries of genetically hypertensive rats: Its possible role in causing hypertension

  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

A study was made of the vascular protein synthesis in young, genetically hyper tensive rats, mainly in their renal arteries. Some of the rats were treated either with 3.5 mg/kg of phenoxybenzamine (POB) or with 4 mg/kg of propranolol twice daily, from 6 to 8 weeks of age.

3H-proline was injected intravenously into each rat before sacrifice to analyse thein vivo incorporation rates of tritiated proline into the vascular collagen and vascular non-collagenous proteins.

Evidence has been presented that: (1) the rates of incorporation of3H-proline into collagen and non-collagenous proteins of the renal arteries in spontaneously hypertensive rats (SHR) and stroke-prone spontaneously hypertensive rats (SHRSP) were greater than those in normotensive Wistar Kyoto rats (WKY); (2) the administration of POB decreased incorporation of3H-proline into the collagen and non-collagenous proteins of the renal arteries concomitant with a reduction of blood pressure in every rat strain; (3) the administration of propranolol failed to decrease the incorporation of3H-proline into these connective tissue proteins in the renal arteries and this drug did not reduce blood pressure in every rat strain; (4) the incorporation rates of3H-proline into these protein fractions in hearts were similar in every rat strain which received any drug treatment.

These results indicate that an increased synthesis of collagen and non-collagenous proteins in the renal arteries of young SHR and SHRSP rats participates in the pathogenesis of spontaneous hypertension in the early hypertensive stage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Armstrong, M. L., Megan, M. B.: Arterial fibrous proteins in Cynomolgus monkeys after atherogenic and regression diets:Circ. Res., 36, 156 (1975).

    Google Scholar 

  2. Fitch, S. M., Harkness, M. L. R., Harkness, R. D.: Extraction of collagen from tissues.Nature, 176, 163 (1955).

    Google Scholar 

  3. Folkow, B.: The hemodynamic consequence of adaptive structural changes of the resistance vessels in hypertension.Clin. Sci., 41, 1 (1971).

    Google Scholar 

  4. Katholi, R. E., Winternitz, S. R., Oparil, S.: Decrease in peripheral sympathetic nervous system activity following renal denervation or unclipping in the one-kidney one-clip Goldblatt hypertensive rat.J. Clin. Invest., 69, 55 (1982).

    Google Scholar 

  5. Kawabe, K., Watanabe, T. X., Shiono, K., Sokabe, H.: Role of the kidney in the pathogenesis of SHR, and other hypertensive rats determined by renal isografts.Jap. Heart J. 20, [Suppl. 1], 87 (1979).

    Google Scholar 

  6. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent.J. Biol. Chem., 193, 265 (1951).

    Google Scholar 

  7. Nakada, T., Lovenberg, W.: Lysine incorporation in vessels of spontaneously hypertensive rats: effects of adrenergic drugs.Eur. J. Pharmacol., 48, 87 (1978).

    Google Scholar 

  8. Nakada, T., Katayama, T., Shimazaki, J.: Suppression of3H-lysine incorporation into the vascular non-collagenous protein in rats treated with estradiol-17β.Eur. J. Pharmacol., 59, 31 (1979).

    Google Scholar 

  9. Nakada, T., Yamori, Y.: The role of vascular protein and renin in chronic two-kidney, one-clip Goldblatt hypertension.Clin. Exp. Hyper., A4 (3), 477 (1982).

    Google Scholar 

  10. Okamoto, K., Yamori, Y., Nagaoka, A.: Establishment of the stroke-prone spontaneously hypertensive rats (SHR).Circ. Res. 34–35 [Suppl. 1], 143 (1974).

    Google Scholar 

  11. Olczyk, K., Dróż, Dż. M., Piwowarczyk, P.: Elastin and collagen metabolism in the arterial wall of rats fed an atherogenic diet.Exp. Pathol., 21, 221 (1982).

    Google Scholar 

  12. Ooshima, A., Fuller, G. C., Cardinale, G. J., Spector, S., Udenfriend, S.: Increased collagen synthesis in blood vessels of hypertensive rats and its reversal by antihypertensive agents.Proc. Natl. Acad. Sci. USA,71, 3019 (1974).

    Google Scholar 

  13. Pfeffer, J. M., Pfeffer, M. A., Frohlich, E. D.: Validity of an indirect tail-cuff method for determining systolic arterial pressure in unanesthetized normotensive and spontaneously hypertensive rats.J. Lab. Clin. Med., 78, 957 (1971).

    Google Scholar 

  14. Skosey, J. L., Zak, R., Martin, A. F., Aschenprenner, V., Rabinowitz, M.: Biochemical correlates of cardiac hypertrophy. V. Labelling of collagen, myosin, and nuclear DNA during experimental myocardial hypertrophy in the rat.Circ. Res., 31, 145 (1972).

    Google Scholar 

  15. Yamabe, H., Lovenberg, W.: Increased incorporation of14C-lysine into vascular protein of the spontaneously hypertensive rats.Eur. J. Pharmacol., 21, 109 (1974).

    Google Scholar 

  16. Yamori, Y., Nakada, T., Lovenberg, W.: Effect of antihypertensive therapy on lysine incorporation into vascular protein of the spontaneously hypertensive rat.Eur. J. Pharmacol., 38, 349 (1976).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan

    Teruhiro Nakada

Authors
  1. Teruhiro Nakada
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakada, T. Enhanced protein synthesis in the renal arteries of genetically hypertensive rats: Its possible role in causing hypertension. International Urology and Nephrology 17, 71–78 (1985). https://doi.org/10.1007/BF02089405

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02089405

Keywords

Search

Navigation