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Gibberellin-like activity in bleeding-sap of root systems ofHelianthus annuus detected by a new dwarf pea epicotyl assay and other methods

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Summary

Gibberellin-like activity was found to be present in bleeding-sap obtained from root systems ofHelianthus annuus. The presence of activity was detected with a newly described simple and sensitive dwarf pea epicotyl assay, and by the barley endosperm and dwarf maize (Zea mays) tests.

The gibberellin-like substance, or substances, are acidic and ethyl acetate soluble, having anR f value of 0.6–0.7 in paper chromatography with isopropyl alcohol: ammonia: water (10∶1∶1 v/v).

The quantity of gibberellin-like activity produced by the roots was estimated at approximately 0.05 μg GA3-equivalents/plant/day.

Other evidence for gibberellin synthesis in roots is discussed, together with the possibility that shoot and root have significant inter-relationships with regard to the production and utilisation of other types of growth hormone.

Zusammenfassung

Am Blutungssaft des Wurzelsystems vonHelianthus annuus wurde ein gibberellinartiges Wirkungsvermögen festgestellt. Das Vorhandensein dieser Aktivität wurde mittels eines neubeschriebenen einfachen und empfindlichen Tests an Zwergerbsen-Epikotylen sowie mit dem Gerstenendosperm- und dem Zwergmais-Test entdeckt.

Die gibberellinartigen Stoffe haben Säurecharakter und sind in Äthylacetat löslich. IhrR f -Wert wurde papierchromatographisch mit Isopropanol: Ammoniak: Wasser=10∶1∶1 v/v als Laufmittel zu 0,6–0,7 ermittelt.

Das Maß der von der Wurzel produzierten gibberellinartigen Aktivität lag bei etwa 0,05 μg GH3-Äquivalenten pro Pflanze und Tag.

Andere Hinweise auf eine Gibberellinsynthese in Wurzeln werden besprochen und ferner die Möglichkeit, daß in Sproß und Wurzel eine signifikante Wechselbeziehung hinsichtlich der Produktion und Verwendung anderer Wuchsstoffarten besteht.

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  1. Department of Botany, University College of Wales, Aberystwyth, Cardiganshire, UK

    I. D. J. Phillips & R. L. Jones

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  1. I. D. J. Phillips
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  2. R. L. Jones
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Phillips, I.D.J., Jones, R.L. Gibberellin-like activity in bleeding-sap of root systems ofHelianthus annuus detected by a new dwarf pea epicotyl assay and other methods. Planta 63, 269–278 (1964). https://doi.org/10.1007/BF02089315

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