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Changes in the quantity of ribosomes in healthy and virus-diseased plants during senescence

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Abstract

The ribosome and virus contents in potato virus X-infected tobacco plants was determined chromatographically. The data were compared with those obtained from measurements of ribosome concentrations in healthy plants of the same age. Bean plants with white clover mosaic virus, and potato and tobacco plants with potato virus X, and tobacco mosaic virus alone and in complex, were similarly tested.

The ribosome content of healthy bean plants was about ten times that of healthy tobacco plants, and of healthy potato plants about 2.5 times that of tobacco plants.

Potato virus X induced a larger increase in ribosome content in tobacco plants than in potato plants. White clover mosaic virus had almost no influence on the ribosome level in bean plants.

During senescence the increase in ribosome and virus contents induced by virus infection declined. In seven-week-old tobacco plants and ten-week-old potato plants the quantity of ribosomes remained constant. Tobacco mosaic virus reduced the increase of ribosome content caused by potato virus X.

Samenvatting

Het gehalte aan ribosomen en virus van tabaksplanten van verschillende leeftijden, geïnoculeerd met aardappelvirus-X werd chromatografisch bepaald en vergeleken met op overeenkomstige wijze bepaalde ribosoomgehalten van even oude gezonde planten. Dezelfde analysen werden uitgevoerd met boneplanten, geïnoculeerd met witte-klavermozaïekvirus, en met aardappelplanten en tabaksplanten, geïnoculeerd met aardappelvirus-X en tabaksmozaïekvirus zowel afzonderlijk als in complex.

Het ribosoomgehalte van gezonde boneplanten bleek meer dan tien keer zo hoog als dat van gezonde tabaksplanten (Tabel 1) en van aardappelplanten ongeveer 2,5× zo hoog als dat van gezonde tabaksplanten (Tabel 2).

Aardappelvirus-X had een verhoging van het ribosoomgehalte bij tabak tot gevolg (Tabel 1). Bij aardappel was deze verhoging geringer (Tabel 2). Witte-klavermozaiekvirus verhoogde het ribosoomgehalte bij bonen nauwelijks (Tabel 1).

Bij veroudering nam de verhoging door virusinfectie sterk af. Bij tabak van zeven weken oud was geen verhoging merkbaar. Bij aardappel trad de verhoging niet meer op, wanneer de planten tien weken oud waren (Tabel 2).

Tabaksmozaïekvirus verminderde de verhoging van het ribosoomgehalte dat door aardappelvirus-X werd veroorzaakt (Tabel 4).

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References

  • Bonner, J., 1965. Membrane-bound ribosomes. In: J. Bonner & J. E. Varner (Ed.), Plant Biochemistry, pp. 34–35. Academic Press, New York & London.

    Google Scholar 

  • Ecklund, P. R. & Moore, T. C., 1969. RNA and protein metabolism in senescent shoot apices of Alaska peas. Amer. J. Bot. 56:327–334.

    Google Scholar 

  • Eskarous, J. K. & Naguib, M. J., 1964. Carbohydrate and nitrogen content of a virus-diseased tobacco plant. Experientia 20:618–619.

    PubMed  Google Scholar 

  • Krzymánska, J. & Hoppe, W., 1969. Biochemical changes in potatoes infected with virus X and X+Y. Biul. Inst. Ochr. Róslin, 44:147–155.

    Google Scholar 

  • Mc. Carthy, D., Jarvis, B. C. & Thomas, B. J., 1970. Changes in the ribosomes extracted from Mung beans infected with a strain of tobacco mosaic virus. J. gen. Virol. 9:9–17.

    PubMed  Google Scholar 

  • Meister, C. W., 1966. Quality and quantity of ribosomes extracted from healthy and from southern bean mosaic virus-infected bean leaves. Phytopathology 56:889.

    Google Scholar 

  • Randles, J. W. & Coleman, D. F., 1970. Loss of ribosomes inNicotiana glutinosa L. infected with lettuce necrotic yellows virus. Virology 41:459–464.

    PubMed  Google Scholar 

  • Reddi, K. K., 1964. Studies on the formation of tobacco mosaic virus ribonucleic acid. IV. Rate of synthesis of virus-induced proteins and ribonucleic acid following infection. Proc. nat. Acad. Sci. U.S.A. 51:619–624.

    Google Scholar 

  • Reid, M. S. & Matthews, R. E. F., 1966. On the origin of the mosaic induced by turnip yellow mosaic virus. Virology 28:563–570.

    PubMed  Google Scholar 

  • Schumann, K., 1970. Untersuchungen über den Proteingehalt virusinfizierter Futtergräser. Archiv. Pfl. schutz 6:435–440.

    Google Scholar 

  • Solecka, M., Profic, H. & Millikan, D. F., 1969. Biochemical patterns in leaf tissue from virus-infected and disease-free apple. IV. Protein changes associated with Spy 227 lethal virus infection. Phytopath. Z. 64:90–93.

    Google Scholar 

  • Venekamp, J. H., Mosch, W. H. M. & Taborsky, V., 1973. Isolation of potato virus X, white clover mosaic virus, tobacco mosaic virus and ribosomes by column chromatography. J. Chromat. 75:235–246.

    Google Scholar 

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Author notes

  1. V. Taborsky (Fellow International Agricultural Centre, Wageningen)

    Present address: Agronomické fakulty, Vysoká Skola Zemědělska V Praze, Katedra Ochrany Rostlin, Suchdol u. Prahy, Ceskoslovakia

  2. J. H. Venekamp

    Present address: Instituut voor Plantenziektenkundig Onderzoek (IPO), Binnenhaven 12, Wageningen, the Netherlands

Authors and Affiliations

  1. Agronomické fakulty, Vysoká Skola Zemědělska V Praze, Katedra Ochrany Rostlin, Suchdol u. Prahy, Ceskoslovakia

    J. H. Venekamp

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  1. J. H. Venekamp
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  2. V. Taborsky
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Venekamp, J.H., Taborsky, V. Changes in the quantity of ribosomes in healthy and virus-diseased plants during senescence. Netherlands Journal of Plant Pathology 79, 62–69 (1973). https://doi.org/10.1007/BF02083667

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