Summary
The present paper deals with the biochemical analysis in the form of quantitative estimation of carbohydrates, proteins, nucleic acids, minerals, ash and moisture of the pollen of two allergenic plants, i.e.Manhuca indica andSpathodea campanulata. Carbohydrates constitute the major dry matter fractions in both the pollen types with highest amount inM. indica (25.37%). Soluble proteins were estimated along with lipid content.S. campanulata showed 12.74% protein andM. indica 11.58%. The estimation of nucleic acids revealed the presence of DNA from 0.36 to 0.72% and RNA from 0.28 to 1.03%. Minerals were found in very low amounts ranging from 0.14 to 0.19% for Calcium and 0.15 to 0.20% for Magnesium. The ash and moisture content were also found variable. The present findings revealed that these chemical constituents may be responsible for the control of the rate and type of allergic reaction caused by the pollen of these two plants.
References
Anderson R.J., Kulp W.L., (1922) —Analysis and composition of corn pollen - preliminary report. Jour. Biol. Chem.,50433–453.
Barrow L., Simpson E.C., (1962) —An EDTA method for the direct routine determination of calcium and magnesium in soils and plant tissue. Soil Sci. Soc. Amer. Proc.,26443–445.
Crook E.M., Jhonston I.R., (1962) —The qualitative analysis of the cell walls of selected species of fungi. Biochem. Jour.,83325–331.
Datta B.K., (1991) —Biosystematic studies on some plants of Birbhum district. Ph. D. Thesis, Visva-Bharati, Santiniketan, India.
Dische Z., (1930) —Mikrocheme 8:4. AOAC, 1980.Official methods of analysis. 13th Ed. AOAC. Washington DC, pp 1018.
Folch J., Lees M., Sloane-Stanley G.H., (1957) —A simple method for the isolation and purification of total lipids from animal tissues. Jour. Biol. Chem.,226497–509.
Itoh L., Kaneko H., (1974) —Yeast lipids in species variation. I. Simple method for estimating the cellular lipids. Yakagaku,23 (6350–354.
Laserna G., Manalo J.B., (1968) —Free amino acid determination of local airborne allergenic pollen extracts by thin layer chromatography. Phillipp. Jour. Sci.,97 (4315–327.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., (1951) —Protein measurement with folin-phenol reagent. Jour. Biol. Chem.,193265–275.
Mandal S., (1987) —A contribution to the environmental biopollutants of West Bengal with reference to allergic disorders. In: Plant Science Research, Bhattacharya G.N., Mandal S. eds., Visva-Bharati Publ., Santiniketan, 217–230.
Mandal S., Chanda S., (1981) —Aeroallergens of West Bengal in the context of environmental pollution and respiratory allergy. Biol. Memoirs,6 (11–61.
Mandal S., Chanda S., (1984) —Aerobiology and allergy. Sci. & Cult.,50 (249–52.
Mandal S., Yonzone R., (1990) —Impact of environmental biopollutants on human helath in Darjeeling. In: Impacts of Environment on Animals and Aquaculture, Manna G.K., Jana B.B. eds., Kalyani Univ. Publ., 327–333.
Mejbaum W., (1939) —Estimation of RNA by Orcinol method. Jour. Physiol. Chem.,258117–123.
Morris D.L., (1948) —The qualitative determination of carbohydrate with drywoods anthrone reagent. Science,107254–255.
Todd F.E., Bretherick O., (1942) —The composition of pollen. Jour. Econ. Entomol.,35312–317.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mandal, S., Datta, B.K. & Bhattacharyya, G.N. Biochemical analysis of the allergenic pollen ofMadhuca indica Gmel. andSpathodea campanulata Beauv. Aerobiologia 9, 197–200 (1993). https://doi.org/10.1007/BF02066262
Issue Date:
DOI: https://doi.org/10.1007/BF02066262