Skip to main content

Advertisement

SpringerLink
Log in

Simultaneous determination of blood flow in cortical bone, marrow, and muscle in canine hind leg by femoral artery catheterization

  • Original Papers
  • Published:
Calcified Tissue Research Aims and scope Submit manuscript

Abstract

The purpose of this study was to develop a repeatable non-destructive method for measurement of bone blood flow in the tibia of the dog. Flow was measured by the residue function technique utilizing [125I]iodoantipyrine (IAP). The percentages of IAP in bone (both cortex and marrow) and in muscle were relatively constant during the 15-minute washout period. From 16 washout curves obtained by femoral artery catheterization, mean (±SE) blood flow (in ml/ml tissue per min) was 0.0159±0.0010 for cortical bone, 0.067±0.004 for adipose marrow, and 0.0816±0.0051 for muscle.

Résumé

Le but de cette étude a consisté à mettre au point une méthode non-destructive et reproductible pour mesurer le débit sanguin osseux au niveau du tibia du chien. Le débit est mesuré par la technique de fonctionnement des résidues en utilisant (125I) iodoantipyrine (IAP). Les pourcentages d'IAP dans l'os (à la fois le cortex et la moëlle) et dans le muslce sont relativement constants pendant une période de lavage de 15 minutes. A partir de 16 courbes de lavage, obtenues par cathétérisation de l'artère femorale, le débit sanguin (en ml/ml de tissu par min) moyen (ISE) est de 0.0159±0.0010 pour l'os cortical, 0.067±0.004 pour la moëlle graisseuse et 0.0816±0.0051 pour le muscle.

Zusammenfassung

Der Zweck dieser Arbeit war, eine wiederholbare, nicht destruktive Methode zur Messung der Blutströmungsverhältnisse in der Tibia beim Hund zu entwickeln. Die Strömung wurde mittels der Residualfunktionen Technik, unter Verwendung von (125J)-Jodantipyrin (IAP) gemessen. Die prozentualen Anteile von IAP im Knochen (Cortex und Mark) und im Muskel waren während der 15-minütigen Auswachperiode relativ konstant. Die Auswertung der Kurven von 16 Auswaschversuchen, die durch Katheterisierung der Femoralarterie gewonnen wurden, ergaben eine mittlere Blutströmungsgeschwindigkeit (±SE in ml/ml Gewebe pro Minute) von 0.0159±0.0010 im kortikalen Knochen; von 0.067±0.004 im Fettmark und von 0.0816±0.0051 im Muskel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allen, T. H., Krzywicki, H. J., Roberts, J. E.: Density, fat, water and solids in freshly isolated tissues. J. appl. Physiol.14, 1005–1008 (1959)

    PubMed  Google Scholar 

  2. Altman, P. L., Dittmer, D. S.: Blood and other body fluids, p. 349. Washington, D.C.: Federation of American Societies for Experimental Biology 1961

    Google Scholar 

  3. Altman, P. L., Dittmer, D. S.: Biology data book, vol. 1, Second ed., p. 393. Bethesda: Federation of American Societies for Experimental Biology 1972

    Google Scholar 

  4. Barnes, D. W. H., Bishop, M., Harrison, G. E., Sutton, A.: Comparison of the plasma concentration and urinary excretion of strontium and calcium in man. Int. J. Radiation Biol.3, 637–646 (1961)

    Google Scholar 

  5. Brookes, M.: Blood flow rates in compact and cancellous bone, and bone marrow. J. Anat. (Lond.)101, 533–541 (1967)

    Google Scholar 

  6. Brookes, M.: Arteriolar blockade: a method of measuring blood flow rates in the skeleton. J. Anat. (Lond.)106, 557–563 (1970)

    Google Scholar 

  7. Copp, D. H., Shim, S. S.: Extraction ratio and bone clearance of Sr85 as a measure of effective bone blood flow. Circulat. Res.16, 461–467 (1965)

    PubMed  Google Scholar 

  8. Domenech, R. J., Hoffman, J. I. E., Noble, M. I. M., Saunders, K. B., Henson, J. R., Subijanto, S.: Total and regional coronary blood flow measured by radioactive microspheres in conscious and anesthetized dogs. Circulat. Res.25, 581–596 (1969)

    PubMed  Google Scholar 

  9. Edholm, O. G., Howarth, S., McMichael, J.: Heart failure and bone blood flow in osteitis deformans. Clin. Sci.5, 249–260 (1945)

    Google Scholar 

  10. Frederickson, J. M., Honour, A. J., Copp, D. H.: Measurement of initial bone clearance of Ca45 from blood in the rat (abstract). Fed. Proc.14, 49 (1955)

    Google Scholar 

  11. Geisler, J. Z., Neuman, W. F.: The membrane control of bone potassium. Proc. Soc. exp. Biol. (N.Y.)130, 608–612 (1969)

    Google Scholar 

  12. Huckabee, W. E.: Use of 4-aminoantipyrine for determining volume of body water available for solute dilution. J. appl. Physiol.9, 157–162 (1956)

    PubMed  Google Scholar 

  13. Johnson, L. C.: Morphologic analysis in pathology: the kinetics of disease and general biology of bone. In: Bone biodynamics, H. M. Frost (ed.), p. 550. Boston: Little, Brown & Co. 1964

    Google Scholar 

  14. Kane, W. J.: The determination of blood flow to tissues of the human leg. Curr. Top. Surg. Res.1, 475–483 (1969)

    Google Scholar 

  15. Kane, W. J., Grim, E.: Blood flow to canine hard-limb bone, muscle, and skin: a quantitative method and its validation. J. Bone Jt Surg. A51, 309–322 (1969)

    Google Scholar 

  16. Kelly, P. J.: Comparison of marrow and cortical bone blood flow by125I-labeled 4-iodoantipyrine (I-Ap) washout. J. Lab. clin. Med.81, 497–505 (1973)

    PubMed  Google Scholar 

  17. Kelly, P. J., Janes, J. M.: Microangiographic and histologic studies of vascular anatomy of the femur and tibia distal to femoral and iliac arteriovenous fistulas in dogs. Anat. Rec.162, 255–267 (1968)

    PubMed  Google Scholar 

  18. Kelly, P. J., Yipintsoi, T., Bassingthwaighte, J. B.: Estimation of canine tibial diaphyseal collateral blood flow by125I-antipyrine washout (abstract). J. Lab. clin. Med.74, 889 (1969)

    Google Scholar 

  19. Kelly, P. J., Yipintsoi, T., Bassingthwaighte, J. B.: Blood flow in canine tibial diaphysis estimation by iodoantipyrine-125I washout. J. appl. Physiol.31, 38–47 (1971)

    PubMed  Google Scholar 

  20. Laurnen, E. L., Kelly, P. J.: Blood flow, oxygen consumption, carbon-dioxide production, and blood-calcium and pH changes in tibial fractures in dogs. J. Bone Jt Surg. A51, 298–308 (1969)

    Google Scholar 

  21. Lunde, P. K. M., Michelsen, K.: Determination of cortical blood flow in rabbit femur by radioactive microspheres. Acta physiol. scand.80, 39–44 (1970)

    PubMed  Google Scholar 

  22. Michelsen, K.: Hemodynamics in the bone marrow of anemic rabbits with increased hematopoiesis. Acta physiol. scand.77, 52–57 (1969)

    PubMed  Google Scholar 

  23. Oro, L., Wallenberg, L., Rosell, S.: Circulatory and metabolic processes in adipose tissuein vivo. Nature (Lond.)205, 178–179 (1965)

    Google Scholar 

  24. Pasternak, H. S., Kelly, P. J., Owen, C. A., Jr.: Metabolic rate in tibia of normal and thyrotoxic dogs. J. Lab. clin. Med.71, 240–246 (1968)

    Google Scholar 

  25. Ramp, W. K., Neuman, W. F.: Some factors affecting mineralization of bone in tissue culture. Amer. J. Physiol.220, 270–274 (1971)

    PubMed  Google Scholar 

  26. Soberman, R., Brodie, B. B., Levy, B. B., Axelrod, J., Hollander, V., Steele, J. M.: The use of antipyrine in the measurement of total body water in man. J. biol. Chem.179, 31–42 (1949)

    Google Scholar 

  27. Spector, W. S.: Handbook of biological data, p. 295. Philadelphia: W. B. Saunders Co. 1956

    Google Scholar 

  28. Dyke, D. van, Anger, H. O., Yano, Y., Bozzini, C.: Bone blood flow shown with F18 and the positron camera. Amer. J. Physiol.209, 65–70 (1965)

    PubMed  Google Scholar 

  29. Weinman, D. T., Kelly, P. J., Owen, C. A., Jr., Orvis, A. L.: Skeletal clearance of Ca47 and Sr85 and skeletal blood flow in dogs. Proc. Mayo Clin.38, 559–570 (1963)

    PubMed  Google Scholar 

  30. White, N. B., Ter-Pogossian, M. M., Stein, A. H. Jr.: A method to determine the rate of blood flow in long bone and selected soft tissues. Surg. Gynec. Obstet.119, 535–540 (1964)

    PubMed  Google Scholar 

  31. Yipintsoi, T., Bassingthwaighte, J. B.: Circulatory transport of iodoantipyrine and water in the isolated dog heart. Circulat. Res.27, 461–477 (1970)

    PubMed  Google Scholar 

  32. Zieler, K. L.: Equations for measuring blood flow by external monitoring of radioisotopes. Circulat. Res.16, 309–321 (1965)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Mayo Foundation, 200 First Street SW, 55901, Rochester, Minnesota, USA

    Edward C. McElfresh & Patrick J. Kelly M. D.

  2. Mayo Clinic, 200 First Street SW, 55901, Rochester, Minnesota, USA

    Edward C. McElfresh & Patrick J. Kelly M. D.

Authors
  1. Edward C. McElfresh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick J. Kelly M. D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This investigation was supported in part by Research Grant AM-15980 from the National Institutes of Health, Public Health Service.

Rights and permissions

Reprints and permissions

About this article

Cite this article

McElfresh, E.C., Kelly, P.J. Simultaneous determination of blood flow in cortical bone, marrow, and muscle in canine hind leg by femoral artery catheterization. Calc. Tis Res. 14, 301–307 (1974). https://doi.org/10.1007/BF02060304

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02060304

Key words

Search

Navigation