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Ki -ras point mutation in different types of colorectal carcinomas in early stages

  • Original Contributions
  • Published:
Diseases of the Colon & Rectum

Abstract

PURPOSE: The aim of this study was to elucidate pathways of carcinogenesis in the colon and rectum by investigating Ki-ras point mutation in different types of colorectal carcinomas in the early stage. METHODS: We analyzed rates of Ki-ras codon 12 mutations in 34 small, polypoid-type carcinomas (Tis or T1), 21 superficial-type carcinomas (Tis or T1), and 42 advanced carcinomas (T2, T3, and T4). RESULTS: Frequency of Ki-ras mutations in superficial-type carcinomas was 14.3 percent (3/21), which was significantly lower than 50 percent (17/34) in small polypoid carcinomas and 40.5 percent (17/42) in advanced carcinomas. These data suggest that another pathway of colorectal carcinogenesis that does not involve Ki-ras point mutation might exist. Among the 17 small polypoid carcinomas with Ki-ras point mutation in which both adenomatous and carcinomatous tissue were examined, 12 showed a mutation of the same type in both carcinomatous and adenomatous tissues. In two cases, mutation was present only in carcinomatous tissue and not in adenomatous tissue; in the other three cases, Ki-ras point mutation was present only in adenomatous tissue but not in carcinomatous tissue. CONCLUSIONS: These data suggest that carcinoma in a small polypoid lesion does not always develop from pre-existing adenoma with Ki-ras point mutation; in a small number of the polypoid-type early carcinomas, polyclonal composition concerning the Ki-ras gene may exist.

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Authors and Affiliations

  1. Department of Surgery, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-Machi, Kawachi-Gun, 329-04, Tochigi, Japan

    M. Kojima M.D.,  F. Konishi M.D., Ph.D., K. Yamashita M.D., Ph.D. & K. Kanazawa M.D., Ph.D.

  2. Second Department of Biochemistry, Jichi Medical School, Tochigi, Japan

    T. Tsukamoto M.D., Ph.D.

Authors
  1. M. Kojima M.D.
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  2. F. Konishi M.D., Ph.D.
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  3. T. Tsukamoto M.D., Ph.D.
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  4. K. Yamashita M.D., Ph.D.
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  5. K. Kanazawa M.D., Ph.D.
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Kojima, M., Konishi, F., Tsukamoto, T. et al. Ki -ras point mutation in different types of colorectal carcinomas in early stages. Dis Colon Rectum 40, 161–167 (1997). https://doi.org/10.1007/BF02054981

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