Abstract
Twenty-one isolates ofAspergillus flavus Link ex Fries obtained from cotton, maize and wheat were screened for their ability to produce aflatoxins on two liquid media. Of these, sixteen isolates were toxigenic and produced only aflatoxin B1 as assessed by bioassay on okra seedlings and TLC method. For screening isolates ofA. flavus for aflatoxin formation, 0.7 % YES+ Salt medium was found to be good as also for obtaining higher yields of the toxin. Isolates ofA. flavus produced aflatoxin B1 ranging from 0.85 to 17.2 mg/50 ml. Maximum yield of aflatoxin was obtained when rice was used as the substrate in case of toxigenic isolates L-27 and C-9, and on maize in isolate M-11.
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References
Burnside, J. E., Sippel, W. L., Forgacs, J., Carll, W. T., Atwood, M. B. &Doll, E. R. (1957) A disease of swine and cattle caused by eating moldy corn II. Experimental production with pure cultures of molds.Am. J. Vet. Res. 18:817–824.
Chohan, J. S. &Gupta, V. K. (1968) Aflaroot disease, a new disease of groundnut caused byAspergillus flavus Link.Indian J. Agri. Sci. 38:568–570.
Davis, M. D., Diener, U. L. &Eldridge, D. W. (1966) Production of aflatoxins B1 and G1 byAspergillus flavus in a semisynthetic medium.Appl. Microbiol. 14:378–380.
Diener, U. L. &Davis, N. D. (1966) Aflatoxin production by isolates ofAspergillus flavus.Phytopathology, 56:1390–1393.
Diener, U. L. &Davis, N. D. (1969) Aflatoxin formation byAspergillus flavus. In “Aflatoxin” (L. A. Goldblatt, ed.), pp. 13–54. Academic Press, New Orleans, Louisiana.
Forgacs, J. &Carll, W. T. (1962) Mycotoxicoses.Advan. Vet. Sci. 7:273–282.
Hartley, R. D., Nesbitt, B. F. &Kelly, J. O. (1963) Toxic metabolites ofAspergillus flavus.Nature, 198:1056–1058.
Hesseltine, C. W., Shotwell, O. L., Ellis, J. J. &Stubble Field, R. D. (1966) Aflatoxin formation byAspergillus flavus.Bact. Rev. 30:795–805.
Indulkar, A. S., Vedanayagam, S. &Rao, S. R. (1971) Seed mycoflora and aflatoxins in cottonseed: A preliminary survey. p. 31–32 (Abstr.) In Second International Symposium on Plant Pathology. Ind. Phytopath. Soc. IARI, New Delhi.
Joffe, A. Z. (1969) Aflatoxin produced by 1626 isolates ofAspergillus flavus from groundnut kernels and soils of Israel.Nature, 221:492.
Kang, M. S. (1970) Pathogenesis in groundnut (Arachis hypogaea L.) byAspergillus flavus Link ex Fries. Ph.D. Thesis, Punjab Agricultural University, Ludhiana.
Koehler, B. (1935) Pathological significance of seed coat injury in Dentcorn. Abstract inPhytopathology, 25:24.
Lillard, H. S., Hanlin, R. T. &Lillard, D. A. (1970) Aflatoxigenic isolates ofAspergillus flavus from pecans.Appl. Microbiol. 19:128.
Maggon, K. K., Viswanathan, L., Venkitasubramanian &Mukerji, K. G. (1969) Aflatoxin production by some Indian strains ofAspergillus flavus Link ex Fries.J. Gen. Microbiol. 59:119–125.
Mateles, R. I. &Adye, J. C. (1965) Production of aflatoxins in submerged culture.Appl. Microbiol. 13:208–211.
Rao, K. S., Madhavan, T. V. &Tulpule, P. G. (1965) Incidence of toxigenic strains ofA spergillus flavus affecting groundnut crop in certain coastal districts of India.Indian. J. Med. Res. 53:1196–1202.
Sargeant, K., Sheridan, A., Kelly, J. O. &Carnaghan, R. B. A. (1961) Toxicity associated with certain samples of groundnut.Nature, 192:1096–1097.
Schroeder, H. W. (1969) Factors influencing the development of aflatoxins in some field crops.J. Stored Prod. Res. 5:187–192.
Schroeder, H. W. &Hein, H. (1967) Aflatoxins: Production of the toxins in vitro in relation to temperature.Appl. Microbiol. 15:441–445.
Sreenivasamurthy, V., Jayarman, A. &Parpia, H. A. B. (1965) Aflatoxin in Indian peanuts: analysis and extraction in “Mycotoxins in Foodstuffs” (G. N. Wogan, ed.), pp. 251–260, M.I.T. Press, Cambridge, Massachusetts.
Taber, R. A. &Schroeder, H. W. (1967) Aflatoxin producing potential of isolates of theAspergillus flavus—oryzae group from peanuts (Arachis hypogaea).Appl. Microbiol. 15:140–144.
Thomas, R. O. (1937) The role of certain fungi in the sick wheat problem.Bim-Bull. Ohio Agriculture Exp. Station, 22 (185):43–45 (Abst. R.A.M. 1937). 16:665).
Tulpule, P. G. (1969) “Aflatoxicoses”.Indian J. Med. Res. (Supplement) 57:36–53.
Wagle, N. G. (1970) Detection and estimation of aflatoxin in groundnuts and groundnut products.IST Bulletin, 22:299–301.
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Mehan, V.K., Chohan, J.S. Aflatoxin B1 producing potential of isolates of Aspergillus flavus Link ex Fries from cotton, maize and wheat. Mycopathologia et Mycologia Applicata 49, 263–274 (1973). https://doi.org/10.1007/BF02050720
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DOI: https://doi.org/10.1007/BF02050720