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Untersuchungen über die immunbiologische Bedeutung löslicher Leberproteine

Studies of the immunbiologic importance of soluble hepatic proteins

  • Published:
Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

For antibodies to mitochondria and microsomes of human and rat liver, kidney and thyroid there was no species- and organspecificity. One part of the antibodies reacted specifically with soluble proteins of human or rat liver tested by different serological methods. The characterization of the soluble hepatic proteins was attempted by ultracentrifugation, gelfiltration and ammoniumsulfate precipitation. The organlinked protein was found to remain in the supernatant at 150000 G of liver homogenates and in the first and second peak after gelfiltration on Sephadex G 100. Upon ammoniumsulfate fractionation most of the activity was precipitated at 45% saturation. It was possible to induce specific immuntolerance to organlinked human or rat hepatic proteins neonatal as well as in adult rabbits. The disturbance of the induced tolerances was possible by altered, not by the nativ proteins. The proteins were altered by coupling to chemically defined haptens (sulfanilic acid) and by heating.

The results provide the possibility to study antigendependent inductions of autoimmunity against hepatic proteins under defined conditions.

Zusammenfassung

Antikörper gegen Mitochondrien- und Mikrosomenfraktionen aus Leber, Niere und Schilddrüse von Mensch und Ratte sind komplett species- und organunspezifisch. Antikörper gegen entsprechende lösliche Proteine der Leber sind unter Anwendung verschiedener serologischer Techniken zum Teil als organ- und im untersuchten Bereich auch als speciesspezifisch anzusprechen. Eine nähere Charakterisierung der löslichen Proteinfraktion gelang mittels Ultrazentrifugation, Chromatographie und Ammoniumsulfatfällung. Die organgebundenen Proteine waren im 150000 G Überstand einer Ultrazentrifugation kernfreier Leberhomogenate enthalten. Chromatographisch fanden sich die Proteine im I. und II. Gipfel einer Gelfiltration an Sephadex G 100. Der Hauptteil dieser Fraktionen ließ sich mit Ammoniumsulfat in 45%iger Anreicherung fällen. Gegen so gewonnene lösliche Leberproteine von Mensch und Ratte waren im Kaninchen neonatal und im Erwachsenenalter unter Beachtung bestimmter Dosiszonen Immuntoleranzen induzierbar. Die erzeugten Toleranzen ließen sich nicht mit nativen, jedoch hitzealterierten und an Sulfanilsäure gekoppelten Proteinen durchbrechen.

Die Versuche bieten die Möglichkeit, antigenbedingte Induktionen einer Autoimmunität gegenüber löslichen Leberproteinen unter definierten Bedingungen zu studieren.

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Literatur

  1. Appelmans, F., andC. de Duve: Tissue fractionation studies;3. Further observations on the binding of acid phosphatase by rat-liver particles. Biochem. J.59, 426–433 (1955).

    PubMed  CAS  Google Scholar 

  2. Asherson, G. L., andD. C. Dumonde: Characterization of auto-antibodies produced in the rabbit by the injection of rat liver. Brit. J. exp. Path.43, 12–20 (1962).

    PubMed  CAS  Google Scholar 

  3. — —: Autoantibodies production in rabbits. V. Comparison of the autoantibody response after the injections of rat and rabbit liver and brain. Immulogy7, 1–8 (1964).

    CAS  Google Scholar 

  4. Bao-Linh, D., G. Hermann etP. Grabar: Etude immunochimique des protéines rénales de la souris. Identification des antigènes organo-specifiques. Ann. Inst. Pasteur106, 670–678 (1964).

    CAS  Google Scholar 

  5. Baker, M. C., D. H. Campbell, S. I. Epstein, andS. J. Singer: Physical chemical studies of soluble antigen-antibody complexes. VII. Thermodynamics of the reaction between benzenearsonic acid-azobivone serum albumin and rabbit antibodies to benzenearsonic acid. J. Amer. chem. Soc.78, 312–318 (1956).

    CAS  Google Scholar 

  6. Behar, A. J., andC. Tal: Experimental liver necrosis produced by the injection of homologous whole liver with adjuvant. J. Path. Bact.77, 591–596 (1959).

    PubMed  CAS  Google Scholar 

  7. Benacerraf, B., andP. G. M. Gell: Immunological specifity of delayed and immediate hypersensitivity reactions. In: Mechanism of cell and tissue damage produced by immune reactions. II. Intern. Sympos. Immunopathology, Brook Lodge 1961, p 136–145. Basel: Schwabe 1962.

    Google Scholar 

  8. Billingham, R. E., andW. K. Silvers: Studies on tolerance of the Y-chromosome antigen in mice. J. Immunol.85, 14–26 (1960).

    PubMed  CAS  Google Scholar 

  9. Beutner, E. H., D. Janian, andE. Witebsky: Serological studies on rabbit anti-bodies to the rabbit anterior pituitary. Immunology7, 172–181 (1964).

    PubMed  CAS  Google Scholar 

  10. Biggs, P. M., andL. N. Payne: Pathological changes following the inoculation of chick embryos with adult cells. I. Spleen cells. Immunology4, 24–37 (1961).

    Google Scholar 

  11. — —: Pathological changes following the inoculation of chick embryos with adult cells. II. Blood cells. Immunology4, 38–48 (1961).

    Google Scholar 

  12. Bigley, N. J., M. C. Dodd, andV. B. Geyer: The immunologic specificity of antibodies to liver ribosomes and nuclei. J. Immunol.90, 416–423 (1963).

    PubMed  CAS  Google Scholar 

  13. Bojrneboe, M., P. Krag, andF. Lundquist: Complement fixation in hepatitis with some lipoids from liver-tissue. Acta path. microbiol. scand.25, 175–176 (1948).

    Google Scholar 

  14. Bollag, W.: Nachweis von organspezifischen Antikörpern. Experientia (Basel)12, 210–211 (1956).

    CAS  Google Scholar 

  15. Bouchier, I. A., K.Rhodes, and S.Sherlock: Serological abnormalities in patients with liver disease. Brit. med. J.1964, 592–594.

  16. Boyd, C.: Fundamentals of immunology. New York: Interscience Publ. 1947.

    Google Scholar 

  17. Boyden, S.: Adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J. exp. Med.93, 107–120 (1951).

    PubMed  CAS  Google Scholar 

  18. Centanui, E.: Über Autocytopräcipitine. Über ein Hepatopräcipitin bei Distomatose. Zbl. Bakt., I. Abt. Orig.43, 508–515 (1907).

    Google Scholar 

  19. Coons, A. H. andM. H. Kaplan: Localization of antigen in tissue cells; improvements in method for detection of antigen by means of fluorescent antibody. J. exp. Med.91, 1–13 (1950).

    PubMed  CAS  Google Scholar 

  20. Cohen, S., G. Otha, E. J. Singer, andH. Popper: Immunocytochemical study of gamma-globulin in liver in hepatitis and postnecrotic cirrhosis. J. exp. Med.111, 285–294 (1960).

    PubMed  CAS  Google Scholar 

  21. Coppo, M., etG. Tedeschi: Essais de provocation expérimentale (rat) d'une hépatite par auto-agression. Gastroenterologie (Basel)7, 39–54 (1964).

    Google Scholar 

  22. D'amelio, V., andP. Perlmann: The distribution of soluble antigens in cellular structures of rat liver. Exp. Cell. Res.19, 383–398 (1960).

    PubMed  Google Scholar 

  23. Dausset, J., andG. Marchall: Cirrhose hepatique avec une substance serique antifoie. In: Immunpathologie. Ist. Internat. Symposion, Basel, p. 113. Basel-Stuttgart: Schwabe 1959.

    Google Scholar 

  24. Dameshek, W.: Theories of Autoimmunity. In: Conceptual advances in Immunology and Oncology. Hoeber Medical Division. New York-Evanston-London: Harpers & Row 1963.

    Google Scholar 

  25. David, J. R., andE. J. Holborow: An attempt to produce immune tolerance to thyroglobulin in rabbits. Lancet1961 I, 83–85.

    Google Scholar 

  26. Davis, B. D., D. H. Moore, E. A. Kabat, andA. Harris: Electrophoretic, ultracentrifugal and immunchemical studies on Wassermann antibody. J. Immunol.50, 1–20 (1945).

    CAS  Google Scholar 

  27. Dobias, G., G. Szecsey, andR. Backhausz: Die immunpathologischen Beziehungen der Leberkrankheiten. Dtsch. med. Wschr.91, 1836–1842 (1966).

    Article  PubMed  CAS  Google Scholar 

  28. Dodd, M. C., N. J. Bigley, V. B. Geyer, F. W. McCoy, andH. E. Wilson: Autoimmune response in rabbits injected with rat and rabbit liver ribosomes. Science137, 688–689 (1962).

    PubMed  CAS  Google Scholar 

  29. Dörner, M., E. J. Simon, andP. A. Miescher: Studies on a liver specific antigen. Fed. Proc.21, 43 (1962).

    Google Scholar 

  30. Drescher, J., andK. Schrader: Titration of poliovirus and influenza virus by means of the hemaggregation test. Amer. J. Hyg.79, 218–235 (1964).

    PubMed  CAS  Google Scholar 

  31. —,A. V. Hennesy, andF. M. Davenport: Photometric methods for the measurement of haemagglutinating viruses and antibody. J. Immunol.89, 794–804 (1962).

    CAS  Google Scholar 

  32. Eaton, M. D., W. D. Murphy, andV. L. Hanford: Heterogenetic antibodies in acute hepatitis. J. exp. Med.79, 539–557 (1944).

    PubMed  CAS  Google Scholar 

  33. Estes, H. R.: Effect of antirat-liver serum on rats. Arch. Path.47, 399–403 (1949).

    CAS  PubMed  Google Scholar 

  34. Farah, F. S., A. K. Kurban, andH. T. Chaglassian: The effects of heat on antibody activity. J. Immunol.93, 300–304 (1964).

    PubMed  CAS  Google Scholar 

  35. Flodin, P., andJ. Killander: Fractionation of human-serum proteins by gel filtration. Biochim. biophys. Acta (Amst.)63, 402–410 (1962).

    CAS  Google Scholar 

  36. Frank, M., andS. Bozsoky: Evaluation of the effect of carbon tetrachloride poisoning on the antigen structure of the rat liver. Kiserl Orvostud.13, 198–204 (1961).

    PubMed  CAS  Google Scholar 

  37. — —: Experiments on the production of a liver specific heterologous immune serum. Z. Immun.-Forsch.119, 110–119 (1960).

    CAS  Google Scholar 

  38. Gajdusek, D. C.: An autoimmune reaction against human tissue antigens in certain acute and chronic diseases. Nature (Lond.)179, 666–668 (1957).

    CAS  Google Scholar 

  39. —: An auto-immune reaction against human tissue antigens in certain acute and chronic diseases. I. Serological investigations. Arch. intern. Med.102, 9–29 (1958).

    Google Scholar 

  40. Gery, I., Y. Yagi, andD. Pressman: Reactions of localizing antikidney antibodies with tissues of various species. J. Immunol.94, 950–958 (1965).

    PubMed  CAS  Google Scholar 

  41. Gabl, F., G. Rietmüller, K. Schumacher u.I. Zeiss: Probleme der Transplantationsimmunologie. Z. Immun.-Forsch.133, 413–455 (1967).

    CAS  Google Scholar 

  42. Gökcen, M.: Auto-immunity in liver disease: serological investigation with clinical correlation. J. Lab. clin. Med.59, 533–541 (1962).

    PubMed  Google Scholar 

  43. Grabar, P., E. Pisi, J. Courcon etG. Lespinats: Analyse immunochimique des constituants solubles de la rate de rat. Ann. Inst. Pasteur107, 749–763 (1964).

    CAS  Google Scholar 

  44. Hase, T., andD. T. Mahin: Electrophoretic and immunoelectrophoteric characterization of soluble cellular proteins in rat liver cell sap. J. Immunol.94, 191–196 (1965).

    PubMed  CAS  Google Scholar 

  45. Hasek: Persönliche Mitteilung 1967.

  46. Hauromitz, F.: Role of the antigen in antibody formation. In: Immunchemie. Berlin-Heidelberg-New York: Springer 1965.

    Google Scholar 

  47. Havens, Jr., W. P.: Bole of auto-immune mechanisme in liver disease. Ann. N.Y. Acad. Sci.104, 1074–1079 (1963).

    Google Scholar 

  48. Henle, W., L. A. Chambers, andV. Groupe: Serological specifity of particulate components derived from various normal mammalian organs. J. exp. Med.74, 495–510 (1941).

    PubMed  CAS  Google Scholar 

  49. Hunter, F. M., R. D. Sparks, R. T. Salzmann: Autoagglutinins in hepatic diseases. Gastroenterology39, 394–403 (1960).

    PubMed  CAS  Google Scholar 

  50. Ischii, K., andS. Yamamoto: Liver cirrhosis and autoallergy. In: Aktuelle Probleme der Hepatologie. Hrsg. vonG. A. Martini u.S. Sherlock. Stuttgart: Thieme 1962.

    Google Scholar 

  51. Jahiel, R. I., andD. Koffler: Hepatic granulomas induced in guinea pigs by Freund's adjuvant with and without homologous liver. Brit. J. exp. Path.42, 338–342 (1961).

    PubMed  CAS  Google Scholar 

  52. Kabat, E. A., andM. M. Mayer: Experimental Immunchemistry. 2nd Ed. Springfeld (Ill.): Thomas 1964.

    Google Scholar 

  53. Kidd, J. G., andW. F. Friedewald: A natural antibody that reacts in vitro with a sedimentable constituent of normal tissue cells. I. Demonstration of the phenomenon. J. exp. Med.76, 543–556 (1942). - II. Specifity of the phenomenon: general discussion. J. exp. Med.76, 557–578 (1942).

    CAS  PubMed  Google Scholar 

  54. Klein, P., u.P. Burkholder: Ein Verfahren zur fluoreszenzoptischen Darstellung der Komplementbindung und seine Anwendung zur histochemischen immunologischen Untersuchung der experimentellen Nierenanaphylaxie. Dtsch. med. Wschr.84, 2001–2004 (1959).

    PubMed  CAS  Google Scholar 

  55. Korngold, L., andD. Pressmann: In vitro purification of tissue localizing antibodies. J. Immunol.71, 1–5 (1953).

    PubMed  CAS  Google Scholar 

  56. Kühn, H. A., u.J. Weinreich: Immunologische Probleme bei chronischer Hepatitis und Lebercirrhose. Dtsch. med. Wschr.89, 723–730 (1964).

    Article  Google Scholar 

  57. Licht, W.: Die Proteine der gesunden menschlichen Leber. Immunoelektrophoretische Untersuchungen. Z. klin. Med.158, 196–207 (1964).

    PubMed  CAS  Google Scholar 

  58. —: Die Proteine der zirrhotischen menschlichen Leber. Immunoelektrophoretische Untersuchungen. Z. klin. Med.158, 208–220 (1964).

    PubMed  CAS  Google Scholar 

  59. —: Zur Frage leberspezifischer Antigene beim Menschen. Klin. Wschr.44, 833–837 (1966).

    PubMed  CAS  Google Scholar 

  60. Mackay, I. D., L. J. Taft, andD. C. Cowling: Lupoid hepatitis. Lancet1956 II, 1323–1326.

    Google Scholar 

  61. Markoff, N., M. Allgöver u.Ch. Kaiser: Zur differentialdiagnostischen Bedeutung der chronischen Hepatitis. In: Akute und chronische Lebererkrankungen. Stuttgart: Thieme 1966.

    Google Scholar 

  62. Meyer zum Büschenfelde, K. H., u.Ch. Schrank: Untersuchungen zur Frage organspezifischer Antigene der Leber. Klin. Wschr.44, 654–656 (1966).

    PubMed  Google Scholar 

  63. —: Immunpathologische Sekundärphänomene bei Lebererkrankungen. In: Akute und chronische Lebererkrankungen. Stuttgart: Thieme 1966.

    Google Scholar 

  64. —, u.H. J. Lange: Statistische Untersuchungen über Zusammenhänge zwischen immunpathologischen Sekundärphänomenen bei Lebererkrankungen. Arch. klin. Med.213, 26–47 (1966).

    PubMed  Google Scholar 

  65. - Über die immunbiologische Spezifität des Leberparenchyms. Experimentelle und klinische Untersuchungen. Habil.-Schr., Mainz 1967.

  66. Metzger, H., andH. Edelhoch: The properties of thyroglobulin. IV. Denaturation kinetics. J. Amer. chem. Soc.83, 1423 (1961).

    CAS  Google Scholar 

  67. Miescher, P. A., A. Braverman u.E. L. Amorosi: Progressive hypergammaglobulinämische Hepatitis. Dtsch. med. Wschr.91, 1525–1532 (1966).

    Article  PubMed  CAS  Google Scholar 

  68. —: Diskussionsbemerkung. In: Akute und chronische Lebererkrankungen. Stuttgart: Thieme 1966.

    Google Scholar 

  69. Milgrom, F., Z. M. Tuggac, andE. Witebsky: Studies on species specificity. J. Immunol.93, 902–909 (1964).

    PubMed  CAS  Google Scholar 

  70. —,W. A. Campebll, andE. Witebsky: Studies on immunization with organspecific haptens. Proc. Soc. exp. Biol. (N.Y.)115, 165–169 (1964).

    CAS  Google Scholar 

  71. —,Z. M. Tüggac, andE. Witebsky: Organspecific antigens of liver, testicle and pituitary. J. Immunol.94, 157–163 (1965).

    PubMed  CAS  Google Scholar 

  72. —, andE. Witebsky: Autoantibodies and autoimmune diseases. J. Amer. med. Ass.181, 706–716 (1962).

    CAS  Google Scholar 

  73. Mitchison, N. A.: Blood transfusion in fowl: An example of immunological tolerance requiring the persistence af antigen. In: Biological Problems of Grafting, p 239. Oxford: Blackwell 1959.

    Google Scholar 

  74. —: Tolerance of erythrocytes in poultry: Induction and specificity. Immunology5, 341–358 (1962).

    PubMed  CAS  Google Scholar 

  75. —: Long-term processes in paralysis, in: Mechanisms of Immunological Tolerance, p. 245. Publ. House Czechosl. Acad. Si., Prague 1962.

    Google Scholar 

  76. Mori, W., andT. Suzuta: Auto-immunization and liver cirrhosis. Nature (Lond.)192, 41–42 (1961).

    CAS  Google Scholar 

  77. Müller, W., u.R. Burger: Die Spezies- und Organspezifität heterologer Leberantikörper. Acta hepato-splenol. (Stuttg.)11, 193–207 (1964).

    Google Scholar 

  78. Norkin, S. A., L. S. Gottlieb, andN. Zamcheck: Effects of liver homogenate and Freunds adjuvant on guinea pig liver and other organs. Amer. J. dig. Dis.7, 824–832 (1962).

    PubMed  CAS  Google Scholar 

  79. Ouchterlony, O.: Diffusion-in-gel methods for immunological analysis. Progr. Allergy5, 1–78 (1958).

    Article  CAS  Google Scholar 

  80. Paronetto, F., andH. Popper: Enhanced antibody formation in experimental acute and chronicliver injury produced by carbon tetrachloride or allyl alcohol. Proc. Soc. exp. Biol. (N.Y.)116, 1060–1064 (1964).

    CAS  Google Scholar 

  81. —,F. Schaffner, R. D. Mutter, J. C. Kniffen, andH. Popper: Circulating antibodies to bile ductular cells in various liver diseases. J. Amer. med. Ass.187, 503–506 (1964).

    CAS  Google Scholar 

  82. — —, andH. Popper: Immunicytochemical reaction of serum of patients with hepatic diseases with hepatic structures. Proc. Soc. exp. Biol. (N.Y.)106, 216–219 (1961).

    CAS  Google Scholar 

  83. Pike, R. M., andM. L. Schulze: The relative heat stability of antibodies in Chromatographie fractions of rabbit antisera to various antigens. J. Immunol.94, 31–36 (1965).

    PubMed  CAS  Google Scholar 

  84. Popper, H., andF. Schaffner: Liver structure and Function. Stuttgart: Thieme 1961.

    Google Scholar 

  85. —: Possible role of immune processes in the self-perpetuation of liver diseases. In: II. Internat. Symposion on Immunpathology. Mechanisms of cell and tissue damage producted by immune reactions, Brook Lodge (Mich.). Ed.P. Grabar, andP. Miescher, p. 303. Basel-Stuttgart: Schwabe 1961.

    Google Scholar 

  86. —,F. Paronetto, andE. Rubin: Piece-meal necrosis as a reflection of immunologic hepatic injury. In: Aktuelle Probleme der Hepatologie. Hrsg.G. A. Martini u.S. Sherlock, S. 83, Stuttgart: Thieme 1962.

    Google Scholar 

  87. Porath, J.: Gel filtration of proteins, peptides and amino acids. Biochim. biophys. Acta (Amst.)39, 193–207 (1960).

    CAS  Google Scholar 

  88. Richterich, R.: Klinische Chemie (Theorie und Praxis). Frankfurt a. M.: Akademische Verlagsgesellschaft 1965.

    Google Scholar 

  89. Sargent, A. K., J. Myers, andM. Richter: The immune response to circulating autolous hepatocellular antigens. J. Immunol.96, 268–272 (1966).

    PubMed  CAS  Google Scholar 

  90. Scheiffarth, F. H., H. Warnatz: Die Pathogenese der chronischen Hepatitis als immunologisches Problem. Klin. Wschr.43, 473–479 (1965).

    PubMed  CAS  Google Scholar 

  91. Siskind, G. W., andL. Thomas: Studies on the runting syndrome in newborn mice. J. exp. Med.110, 511–523 (1959).

    PubMed  CAS  Google Scholar 

  92. Sulitzeanu, D., Y. Yagi, andD. Pressmann: Separation of an antigenic fraction from rat liver responsible for the formation of liver localizing antibodies. J. Immunol.90, 424–428 (1963).

    PubMed  CAS  Google Scholar 

  93. Schachtschnabel, D., u.W. Zillig: Untersuchungen zur Biosynthese der Proteine. Hoppe-Seylers Z. physiol. Chem.314, 262–275 (1959).

    Google Scholar 

  94. Schaffner, F., andH. Popper: A phagocytic and protein-forming mesenchymal cell in human cirrhosis. Nature (Lond.)196, 684–685 (1962).

    CAS  Google Scholar 

  95. Scheiffarth, F. H., H. Götz, u.G. Thuma: Immunelektrophoretische Studien zur Frage der Identität menschlicher Organ- und Serumproteine. II. Mittlg. Clin. chim. Acta7, 355–365 (1962).

    PubMed  CAS  Google Scholar 

  96. Schierz, G.: Zur antikomplementären Wirkung von Serum und Gammaglobulin. Z. Immun.-Forsch.127, 418–427 (1964).

    CAS  Google Scholar 

  97. Starzl, T. E., H. A. Kaupp, D. R. Brock, andJ. W. Linman: Studies on the rejection of the transplanted homologous dog liver. Surg. Gynec. Obstet.112, 135–144 (1961).

    CAS  PubMed  Google Scholar 

  98. Steffen, C.: Der Antiglobulin-Konsumptionstest. Seine theoretischen Grundlagen, Technik und Anwendungsbereiche. Klin. Wschr.40, 613–622 (1962).

    PubMed  CAS  Google Scholar 

  99. Steiner, J. W., J. S. Carruthers, R. Baumal, andS. R. Kalifat: Experimental immunologic liver injury and the concept of autodestruction. Part I and II. Canad. med. Ass. J.85, 1369–1376, 1425–1436 (1961).

    PubMed  CAS  Google Scholar 

  100. —,B. Langer, andD. L. Schatz: The local and systematic effects of Freund's adjuvant and its fractions. Arch. Path.70, 424–434 (1960).

    PubMed  CAS  Google Scholar 

  101. —: Investigations of allergic liver injury. Amer. J. Path.38, 411–436 (1961).

    CAS  PubMed  Google Scholar 

  102. Thorbecke, G. J., andB. Benacerraf: Tolerance in adult rabbits by repeated non-immunogenic doses of bovine serum albumin. Immunology13, 141–145 (1967).

    PubMed  CAS  Google Scholar 

  103. Vogt, P. K.: The immunology of liver microsomes. I. The properties of the quantitative precipitin system. Z. Naturforsch.15B, 213–217 (1960). - II. The immunological relations of microsomes to other cell fractions. Z. Naturforsch.15B, 218-221 (1960).

    CAS  Google Scholar 

  104. Vogt, P. K.: The localization of tissue-specific antigens within the structural components of endoplastic reticulum. Z. Naturforsch.15B, 221–225 (1960).

    CAS  Google Scholar 

  105. Weigle, W. O.: The induction of autoimmunity in rabbits following injection of heterologous or altered homologous thyroglobulin. J. exp. Med.121, 289–308 (1965).

    PubMed  CAS  Google Scholar 

  106. —: Effect of thymectomy on the termination of immunological tolerance in rabbits. Nature (Lond.)201, 632–633 (1964).

    CAS  Google Scholar 

  107. —: The immune response of rabbits tolerant to bovine serum albumin to the injection of other heterologous serum albumin. J. exp. Med.114, 111–125 (1961).

    PubMed  CAS  Google Scholar 

  108. —: Termination of acquired immunological tolerance to protein antigens following immunization with altered protein antigens. J. exp. Med.116, 913–928 (1962).

    PubMed  CAS  Google Scholar 

  109. Weigle, W. O.: The effect of x-radiation and passive antibody on immunologic tolerance in the rabbit to bovine serum albumin. J. Immunol.92, 113–117 (1964).

    PubMed  CAS  Google Scholar 

  110. —: The immune response of BSA tolerant rabbits to injections of BSA following the termination to the tolerant state. J. Immunol.92, 791–797 (1964).

    PubMed  CAS  Google Scholar 

  111. —: Studies on the termination of acquired tolerance to serum protein antigens following injection of serologically related antigens. Immunology7, 239–247 (1964).

    PubMed  CAS  Google Scholar 

  112. Weiler, W.: Die Änderung der serologischen Spezifität von Leberzellen der Ratte während der Cancerogenese durch p-Dimethylaminoazobenzol. Z. Naturforsch.11B, 31–38 (1956).

    CAS  Google Scholar 

  113. Weir, D. M.: Liver autoantibodies in the rat. Immunology6, 581–591 (1963).

    PubMed  CAS  Google Scholar 

  114. Whitbeck, E. G., andL. T. Rosenberg: Antigenic properties of microsomes from guinea pig spleen, liver and lymphnodes. Immunology7, 363–374 (1964).

    PubMed  CAS  Google Scholar 

  115. Wiedermann, G., M. Dörner u.P. A. Miescher: Autoimmunitäre Vorgänge gegen Lebergewebe. Schweiz. med. Wschr.94, 257–262 (1964).

    PubMed  CAS  Google Scholar 

  116. Wigand, R.: Eine einfache Methode zur Beseitigung der antikomplementären Aktivität von Seren. Z. Immun.-Forsch.124, 368–369 (1962).

    CAS  Google Scholar 

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  1. II. Medizinische Klinik und Poliklinik der Universität Mainz, Deutschland

    K. -H. Meyer zum Büschenfelde

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

Theodor Frerichs-Preis 1968.

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Meyer zum Büschenfelde, K.H. Untersuchungen über die immunbiologische Bedeutung löslicher Leberproteine. Z. Gesamte Exp. Med. 148, 131–163 (1968). https://doi.org/10.1007/BF02044245

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