Summary
Mitochondrial glycerophosphate-acyltransferase activity (GPAT) was determined in the isolated and perfused liver of diet-induced hyperlipemic rats, and was found to be significantly increased compared to normal rats. A positive correlation existed between hepatic triglyceride output and GPAT. Perfusion of 10−5 M glucagon induced a significant reduction in GPAT levels. It is suggested that the lipid-lowering action of glucagon may be mediated also through an inhibition of GPAT activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aas M., Daae L. N. W.: Fatty acid activation and acyl transferase in organs from rats in different nutritional states - Biochim. biophys. Acta (Amst.)239, 208, 1971.
Assimacopoulos-Jeannet F., Singh A., Le Marchand Y., Loten E. G., Jeanrenaud B.: Abnormalities in lipogenesis and triglyceride secretion by perfused livers of obese-hyperglycaemic (ob/ob) mice: relationship with hyperinsulinemia - Diabetologia10, 155, 1974.
Bates E. J., Topping D. L., Sooranna S. P., Saggerson D., Mayes P. A.: Acute effects of insulin on glycerol-phosphate acyltransferase activity, ketogenesis and serum free fatty acid concentration in perfused rat liver - FEBS Letters84, 225, 1977.
Bates E. J., Saggerson D.: A selective decrease in mitochondrial glycerophosphate acyltransferase activity in livers from streptozotocin-diabetic rats - FEBS Letters84, 229, 1977.
Bremer J., Christiansen R. Z., Borrebaek B.: Control of beta-oxidation - Biochem. Soc. Trans.6, 83, 1978.
Christiansen R. Z.: Regulation of palmitate metabolism by carnitine and glucagon in hepatocytes isolated from fasted and carbohydrate refed rats - Biochim. biophys. Acta (Amst.)488, 249, 1977.
Duhault J., Beregi L., Bolanger M.: Pharmacological effects of 780SE (992) on hyperlipidemic and obese rats - Postgrad. med. J.51 (Suppl. 1), 99, 1975.
Duncombe W. G.: Colorimetric micro-determination of non-esterified fatty acid in plasma - Clin. chim. Acta9, 122, 1964.
Fallon H. J., Kemp E. L.: Effects of diet on hepatic triglyceride synthesis - J. clin. Invest.47, 712, 1968.
Fallon H. J., Lamb R. G., Jamdar S. C.: Phosphatidate phosphohydrolase and the regulation of glycerolipid biosynthesis - Biochem. Soc. Trans.5, 37, 1977.
Faloona G. R., Unger R. H.: Glucagon - In:Jaffe B. M., Behrmann H. R. (Eds.): Method of hormone radioimmunoassay. Academic Press, New York, 1974; p. 317.
Folch J., Lees M., Sloane-Stanley G. H.: A simple method for the isolation and purification of total lipids from animal tissues - J. biol. Chem.226, 497, 1957.
Heimberg M., Weinstein I., Kohout M.: The effects of glucagon, dibutyryl cyclic adenosine 3′,5′ monophosphate, and the concentration of free fatty acid on hepatic lipid metabolism - J. biol. Chem.244, 5131, 1969.
Herbert V., Kam-Seng L., Gottlieb C. W., Bleicher J.: Coated charcoal immunoassay of insulin - J. clin. Endocr.25, 1375, 1965.
Lamb R. G., Fallon H. J.: An enzymatic explanation for dietary-induced alterations in hepatic glycerolipid metabolism - Biochim. biophys. Acta (Amst.)348, 179, 1974.
Le Marchand Y., Singh A., Assimacopoulos-Jeannet F., Orci L., Roullier C., Jeanrenaud B.: A role for the microtubular system in the release of VLDL by perfused mouse liver - J. biol. Chem.248, 6862, 1973.
Lloyd-Davies K. A., Brindley D. N.: Palmitate activation and esterification in microsomal fractions of rat liver - Biochem. J.152, 39, 1975.
Mangiapane E. H., Lloyd-Davies K. A., Brindley D. N.: A study of some enzymes of glycerolipid biosynthesis in rat liver after subtotal hepatectomy - Biochem. J.134, 103, 1973.
Mayes P. A., Felts J. M.: Regulation of fat metabolism in the liver - Nature (Lond.)215, 716, 1967.
McGarry J. D.: New perspectives in the regulation of ketogenesis - Diabetes28, 517, 1979.
Mellamby J., Williamson D. H.: Hydroxybutyrate - In:Bergmeyer H. V. (Ed.): Methoden der enzymatischen Analyse. 2nd ed. Verlag Chemie, Weinheim, 1971; p. 1172.
Miller L. L.: Technique of isolated rat liver perfusion - In:Bartosek I., Guaitani A., Miller L. L. (Eds): Isolated liver perfusion and its applications. Raven Press, New York, 1973; p. 11.
Nimmo H. G.: The location of glycerol-phosphate acyltransferase and fatty acyl-CoA synthetase on the inner surface of the mitochondrial outer membrane - FEBS Letters101, 262, 1979.
Nosadini R., Soldà G., De Biasi F., Tiengo A.: Metabolic effects of glucagon in endogenous hypertriglyceridemia - Acta diabet. lat.15, 215, 1978.
Nosadini R., Ursini F., Tessari P., Garotti C., Tiengo A., Gregolin C.: Perfused liver carnitine palmitoyl transferase activity and ketogenesis in streptozotocin treated and genetic hyperinsulinemic rats. Effect of glucagon - Hormone metab. Res.11, 674, 1979.
Olsen C.: An enzymatic fluorimetric micro-method for the determination of acetoacetate, hydroxybutyrate, pyruvate and lactate- Clin. chim. Acta33, 293, 1971.
Schade D. S., Eaton R. P.: Modulation of fatty acid metabolism by glucagon in man. I. Effects in normal subjects - Diabetes24, 502, 1975.
Schonfeld G., Pfleger B.: Utilization of exogenous free fatty acids for the production of very low density lipoprotein triglyceride by livers of carbohydrate-fed rats - J. Lipid Res.12, 614, 1971.
Soler-Argilaga C., Russel R. L., Heimberg M.: Enzymatic aspects of the reduction of microsomal glycerolipid biosynthesis after perfusion of the liver with dibutyryl-adenosine 3′,5′ monophosphate - Arch. Biochem.190, 367, 1978.
Weinstein I., Klausner H. A., Heimberg M.: The effect of concentration of glucagon on output of triglyceride, ketone bodies, glucose and urea by liver - Biochim. biophys. Acta (Amst.)296, 300, 1973.
Whiting P. H., Bowley M., Sturton R. G., Pritchard P. H., Brindley D. N., Hawthorne J. N.: The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycero-lipid synthesis in rat liver - Biochem. J.168, 147, 1977.
Williamson J. R., Browning E. T., Thurman R. G., Scholz R.: Inhibition of glucagon effects in perfused rat liver by (+)decanoyl carnitine - J. biol. Chem.244, 5055, 1969.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tessari, P., Meneghel, A., Avogaro, A. et al. Glycerophosphate acyltransferase activity in perfused liver of normal and hyperlipemic rats: Glucagon effect. Acta diabet. lat 18, 357–363 (1981). https://doi.org/10.1007/BF02042820
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02042820