Abstract
During the formation ofHelianthus tuberosus tubers the activities of arginine decarboxylase (ADC) and S-adenosylmethionine decarboxylase (SAMDC), examined in medullary parenchyma cells, increase with the increase in weight of the tuber. The ornithine decarboxylase (ODC) activity is about 100-fold less with respect to ADC activity, and it was detected only during the deceleration phase of the growth curve. Spermidine and spermine content are strictly related to the SAMDC activity and tuber growth. The increase of ADC and SAMDC activity is directly related to cell extension and increase in weight. The limited area of cell division in parenchyma tissue found during the first stage of tuber formation could justify the low ODC activity. The data suggest that ADC affects mainly growth processes, while ODC seems to be preferentially related to cell division.
Similar content being viewed by others
References
Altman A, Friedman R, Levin N (1982) Arginine and ornithine decarboxylases: The polyamine biosynthetic enzymes of Mung bean seedlings. Plant Physiol 69:876–879
Bagni N, Donini A, Serafini-Fracassini D (1972) Content and aggregation of ribosomes during formation, dormancy and sprouting of tubers ofHelianthus tuberosus. Physiol Plant 27:370–375
Bagni N, Malucelli B, Torrigiani P (1980) Polyamines, storage substances and abscisic acid-like inhibitors during dormancy and very early activation ofHelianthus tuberosus tuber tissues. Physiol Plant 49:341–345
Bagni N, Serafini-Fracassini D, Torrigiani P (1981a) In: Caldarera CA, Zappia V, Bachrach V (eds) Advances in polyamine research 3. Raven Press, New York, pp 377–388
Bagni N, Torrigiani P, Barbieri P (1981b) Effect of various inhibitors of polyamine synthesis on the growth ofHelianthus tuberosus. Med Biol 59:403–409
Bagni N, Torrigiani P, Barbieri P (1983)In vitro andin vivo effect of ornithine and arginine decarboxylase inhibitors in plant tissue culture. In: Bachrach V, Kaye AM, Chayen R (eds) Advances in polyamine research 4. Raven Press, New York, pp 409–417
Cocucci S, Bagni N (1968) Polyamine-induced activation of protein synthesis in ribosomal preparation fromHelianthus tuberosus tissue. Life Sci 7:113–120
Cohen E, Malis-Arad S, Heimer YM, Mizrahi Y (1983) The involvement of polyamines in the cell cycle ofChlorella. In: Bachrach U, Kaye AM, Chayen R (eds) Advances in polyamine research 4. Raven Press, New York, pp 449–450
Courduroux JC (1967) Etude du mécanisme physiologique de la tubérisation chez le topinanbour (Helianthus tuberosus L.). Thèse, Masson et Cie (eds) Paris, pp 219–348
Courduroux JC, Gendrand M, Teppar-Misson C (1972) Action comparée de l'acide gibbérellique et du froid sur la repousse et la levée de dormance de tubercules de topinanbour (Helianthus tuberosus L.) cultivés in vitro. Physiol Vég 10:503–514
Dai Y, Galston AW (1981) Simultaneous phytochrome-controlled promotion and inhibition of arginine decarboxylase activity in buds and epicotyls of etiolated peas. Plant Physiol 67:266–269
Dai Y, Kaur-Sawhney R, Galston AW (1982) Promotion by gibberellic acid of polyamine biosynthesis in internodes of light-grown dwarf peas. Plant Physiol 69:103–105
Flores HE, Galston AW (1982) Polyamine and plant stress: Activation of putrescine biosynthesis by osmotic shock. Science 217:1259–1261
Heby O (1981) Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19:1–20
Heimer YM, Mizrahi Y, Bachrach U (1979) Ornithine decarboxylase activity in rapidly proliferating plant cells. FEBS Lett 104:146–148
Kallio A, McCann PP, Bey P (1981) DL-α-(difluoromethyl) arginine: A potent enzyme-activated irreversible inhibitor of bacterial arginine decarboxylases. Biochemistry 20:3163–3166
Kaur-Sawhney R, Shih L, Galston AW (1982) Relation of polyamine biosynthesis to the initiation of sprouting in potato tubers. Plant Physiol 69:411–415
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Montague MJ, Armstrong TA, Jaworski EG (1979) Polyamine metabolism in embryogenic cells ofDaucus carota. II. Changes in arginine decarboxylase activity. Plant Physiol 63:341–345
Serafini-Fracassini D, Bagni N, Cionini PG, Bennici A (1980) Polyamines and nucleic acids during the first cell cycle ofHelianthus tuberosus tissue after the dormancy break. Planta 148:332–337
Smith TA (1963) L-arginine-carboxyl-lyase of higher plants and its relation to potassium nutrition Phytochem 2:241–252
Smith TA (1979) Arginine decarboxylase of oat seedlings. Phytochem 18:1447–1452
Smith TA, Sinclair C (1967) The effect of acid feeding on amine formation in barley. Ann Bot 31:103–111
Smith TA, Best GR (1977) Polyamines in barley seedlings. Phytochem 16:841–843
Suresh MR, Adiga PR (1977) Putrescine-sensitive (artifactual) and insensitive (biosynthetic) Sadenosyl-L-methionine decarboxylase activities ofLathyrus sativus seedlings. Eur J Biochem 79:511–518
Suzuki Y, Hirosawa E (1980) S-adenosylmethionine decarboxylase of corn seedlings. Plant Physiol 66:1091–1094
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bagni, N., Barbieri, P. & Torrigiani, P. Polyamine titer and biosynthetic enzymes during tuber formation ofHelianthus tuberosus . J Plant Growth Regul 2, 177–184 (1983). https://doi.org/10.1007/BF02042246
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02042246