Skip to main content
SpringerLink
Log in

Studies on the application of microorganisms immobilized by radiation in the pharmaceutical industry

Dehydrogenation of cortisone acetate to prednisone acetate catalyzed by immobilized A. simplex by-2-13

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Immobilization of the bacteria A. simplex by-2-13 was obtained by irradiation polymerization with an electron beam and gamma-radiation at 0°C. Prednisone acetate (P. A.) was derived from cortisone acetate (C. A.) by 1,2-dehydrogenation, catalyzed by immobilized A. simplex by-2-13. The immobilized bacteria were used 10 times in succession with a transformation percentage ranging from 77 to 86% for electron beam radiation and 79 to 99% for gamma radiation. Dehydrogenation, was carried out at pH 7.2, 34°C, 180–200 r.p.m. for 24 hours. 3% (v/v) anhydrous alcohol was added to the system at onset. Scanning electron micrographs documented the structure of the immobilized bacteria. In this work, the effect of absorbable doses of radiation and the reaction time of non-immobilized bacteria on dehydrogenase activity were studied. The effect of pH, temperature and concentration of substrate were also studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. G. GUILBANLT, Handbook, of Enzyme Methods of Analysis, p. 445.

  2. J. C. JOHNSON, Immobilized Enzymes Preparation and Engineering Recent Advances, p. 302.

  3. Advances in Applied Microbiology, Vol. 20, 1976, p. 240.

  4. CHIBATA: The Immobilized Enzymes Japan: Jul. 1974.

  5. 13th Intern. TNO Conf. 27–28 March, 1980, p. 83.

  6. J. H. T. LUONG, M. C. TSENG, Appl. Microbiol. Biotechnol., 19 (1984) 207.

    Google Scholar 

  7. 3rd Intern. Meeting on Radiation Processing, Japan Oct. 1980.

  8. A. HOFFMAN, Radiat. Phys. Chem., 9 (1977) 207.

    Google Scholar 

  9. E. GALAS, S. JEDRZEJCZK, W. PEKALA, M. PIETRZAK, Radiochem. Radianal. Letters, 47 (1980) 355.

    Google Scholar 

  10. M. YOSHIDU, M. KUMAKURA, L. KAETSU, Polymer, 1 (1979) 20.

    Google Scholar 

  11. I. KAETSV, M. KVMAKURA, M. YOSHIDA, Biotechnol. Bioeng., 30 (1979) 863.

    Google Scholar 

  12. M. KVMAKUNA, I. KAETSO, J. Appl. Biochem., (1983) 346.

  13. J. C. JOHNSON, Immobilized Enzymes Preparation and Engineering, Recent Advances, p. 74.

  14. M. KUMAKURA, I. KAETSU, Enzyme Microbiol. Technol., 6 (1984).

  15. M. KOMAKURA, M. YOSHIDA, I. KAETSU, Biotechnol. Bioeng., 30 (1979) 697.

    Google Scholar 

  16. LIR TUNG-FU, Radiat. Phys. Chem., 20, (1982) 202.

    Google Scholar 

  17. K. A. KOSHCHEYENKO, M. V. TVAKIA, C. K. SKRYABIN, Enzyme Microb. Technol. 5 (1983).

Download references

Author information

Authors and Affiliations

  1. Tianjin Technical Physics Institute, Tianjin, (People's Republic of Chica)

    Zhang Zhi-Hui & Yuan Zhi-Gang

Authors
  1. Zhang Zhi-Hui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuan Zhi-Gang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhi-Hui, Z., Zhi-Gang, Y. Studies on the application of microorganisms immobilized by radiation in the pharmaceutical industry. Journal of Radioanalytical and Nuclear Chemistry, Articles 125, 157–164 (1988). https://doi.org/10.1007/BF02041761

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02041761

Keywords

Search

Navigation