Skip to main content
SpringerLink
Log in

Immunochemical parameters of antibodies as possible end-points in evaluating the radiobiological effects associated with chronic exposure to tritium

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Using an adaptation of the radioimmunoassay technique, the present study reports on immunochemical parameters of the antibodies synthesized in vivo against progesterone by rats previously exposed to long-term internal contamination with tritiated water. The antibody concentration of the antisera and the equilibrium constant of the antibody-antigen reaction were measured in vitro. The corresponding dose range of the irradiation was between 6 and 569 mGy. An increase of the affinity constant of the antibodies produced by the irradiated organisms was found for the corresponding doses of up to 60 mGy. The antibody concentration seems to decrease for doses above 30 mGy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. E. ANDERSON, S. TOKUDA, W. L. WILLIAMS, N. L. WARNER, Amer. J. Pathol., 108 (1982) 24.

    Google Scholar 

  2. R. E. ANDERSON, W. L. WILLIAMS, S. TOKUDA, Int. J. Radiat. Biol., 53 (1988) 103.

    Google Scholar 

  3. C. C. STEWART, A. P. STEVENSON, R. C. HABBERSETT, Int. J. Radiat. Biol., 53 (1988) 77.

    Google Scholar 

  4. R. J. HOOGHE, J. R. MAISIN, F. VANDER PLAETSE, J. URBAIN, G. URBAIN-VANSANTEN, Int., J. Radiat. Biol., 53 (1988) 153.

    Google Scholar 

  5. J. A. STRAND, M. P. FUJIHARA, T. M. POSTON, C. S. ABERNETHY, Radiat. Res., 91 (1982) 533.

    PubMed  Google Scholar 

  6. I. INABA, Y. NISHIMURA, H. TOKADA, Y. KASIDO, R. ICHIKAWA, J. Radiat. Res., 22 (1981) 287.

    PubMed  Google Scholar 

  7. I. DOROBANTU, E. IANCU, M. NEAGU, Rev. Roum. Biochim., 27 (1989) 139.

    Google Scholar 

  8. I. PETCU, A. STINGA, Romanian J. Biophys., 3 (1993) 41.

    Google Scholar 

  9. I. DOROBANTU, G. MIHAIESCU, E. IANCU, Patent 83871/27. 01. 1984 OSIM, Romania.

  10. M. W. STEWARD, J. STEENSGAARD, Antibody Affinity: Thermodynamic Aspects and Biological Significance, CRC Press Inc., Boca Raton, Florida, 1983.

    Google Scholar 

  11. SHU-ZHENG LIU, in: Low Dose Irradiation and Biological Defense Mechanisms, T. SUGAHARA, L. A. SAGAN, and T. AOYAMA (Eds), Elsevier Science Publishers B.V., Amsterdam, 1992, p. 225.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Physics and Nuclear Engineering, P.O. Box MG-6, R-76900, Bucharest, (Romania)

    I. Petcu & I. Dorobantu

Authors
  1. I. Petcu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Dorobantu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petcu, I., Dorobantu, I. Immunochemical parameters of antibodies as possible end-points in evaluating the radiobiological effects associated with chronic exposure to tritium. Journal of Radioanalytical and Nuclear Chemistry, Articles 189, 77–82 (1995). https://doi.org/10.1007/BF02040184

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02040184

Keywords

Search

Navigation