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Determination of total, and bound Se in sera by INAA

  • Nuclear Analytical Methods in Biology and Medicine
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Abstract

In this study we report on the comparison between the total selenium in serum (“total Se”) with that which is apparently bound to high molecular weight (>12,000 D) species, presumably proteins (“bound Se”). Nine hundred seventy seven (977) serum samples arising out of a population-based epidemiological study were prepared in duplicate for the determination of “total Se” by pipeting directly into irradiation vials; and separate duplicate aliquots were dialyzed against DI water for the determination of “bound Se”. All samples were analyzed by neutron activation analysis via77mSe (17.4 s). A small dialyzable Se component (∼6%) (“free Se”), defined as the difference between the “total Se” minus the “bound Se”, was identified.

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References

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Authors and Affiliations

  1. Research Reactor Center, Missouri University, 65211, Columbia, MO, USA

    V. L. Spate, M. M. Mason, C. L. Reams, C. K. Baskett, J. S. Morris & D. S. Mills

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  1. V. L. Spate
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  2. M. M. Mason
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  3. C. L. Reams
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  4. C. K. Baskett
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  5. J. S. Morris
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  6. D. S. Mills
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Spate, V.L., Mason, M.M., Reams, C.L. et al. Determination of total, and bound Se in sera by INAA. Journal of Radioanalytical and Nuclear Chemistry, Articles 179, 315–322 (1994). https://doi.org/10.1007/BF02040166

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  • DOI: https://doi.org/10.1007/BF02040166

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