Abstract
For purposes of radioimmunoanalytical determination of serum ferritin, conditions for antigen iodination and separation were searched for, which could provide a satisfactory radiochemical purity and specific activity, high immunoreactivity and stability of the resulting labeled product, necessary for an acceptable expiration of the RIA kit. Two iodination methods (chloramine and conjugation methods) were tested, and a three-step procedure was elaborated for iodination and separation by gel column chromatography. The iodinated antigen obtained —125I-placental ferritin with IRmax of about 80%,125I−<8%, specific activity of about 0.6MBq/μg and stability for the expiration period of 3 to 4 months — is quite satisfactory for the RIA applications.
Similar content being viewed by others
References
S. A. BERSON, R. S. YALOW, J. Clin. Invest., 38 (1959) 1996.
S. A. BERSON, R. S. YALOW, Clin. Chim. Acta, 22 (1968) 51.
G. M. MILES, C. N. HALES, Nature, 219 (1968) 189.
G. ADDISON, M. BEAMIS, C. HALLES, M. HODKINS, A. JACOBS, P. LLEWELLYN, J. Clin. Pathol., 25 (1972) 326.
F. C. GREENWOOD, W. M. HUNTER, J. S. GLOVER, Biochem. J., 89 (1963) 114.
A. E. BOLTON, V. LEE-OWN, R. KRAMER McLEAN, G. S. CHALLAND, Clin. Chem., 25 (1979) 1826.
J. C. CALVO, J. P. RADICELLA, E. H. CHARREAU, Biochem. J., 212 (1983) 259.
C. S. CHIANG, Clin. Chem., 33 (1987) 1245.
A. E. BOLTON, W. M. HUNTER, Biochem. J., 133 (1973) 529.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glagoličová, A., Wernischová, V. & Novák, F. Iodination of placental ferritin for RIA. Journal of Radioanalytical and Nuclear Chemistry, Articles 162, 199–205 (1992). https://doi.org/10.1007/BF02039941
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02039941